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Inhibitory effects of total ginsenoside on bleomycin-induced pulmonary fibrosis in mice

Lu Yang1, Pan-Pan Chen1, Min Luo2

  • 1Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education, Zunyi Medical University, Zunyi, Guizhou, 563006, China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|April 10, 2019
PubMed

Insights

Total ginsenoside (TG), a ginseng component, shows promise in treating pulmonary fibrosis. TG ameliorates bleomycin-induced lung injury in mice by regulating key signaling pathways and matrix metalloproteinases.

Area of Science:

  • Pulmonology
  • Pharmacology
  • Cell Biology

Background:

  • Pulmonary fibrosis, a severe outcome of interstitial lung diseases, lacks effective treatments and has poor prognosis.
  • Total ginsenoside (TG), derived from ginseng, is known to inhibit acute lung injury.

Purpose of the Study:

  • To evaluate the protective effects of TG against pulmonary fibrosis.
  • To elucidate the underlying mechanisms of TG's action in pulmonary fibrosis.

Main Methods:

  • A bleomycin-induced pulmonary fibrosis model was established in BALB/c mice.
  • Mice were treated with varying doses of TG (40, 80, 160 mg/kg/d) for 28 days.
  • Pulmonary fibrosis was assessed via lung coefficient, histological staining (H&E, Masson's trichrome), and alpha-smooth muscle actin expression.
  • The transforming growth factor-β1 (TGF-β1)/Smad signaling pathway and matrix metalloproteinase (MMP) system were analyzed at the gene and protein levels.

Main Results:

  • TG treatment significantly ameliorated bleomycin-induced pulmonary fibrosis in mice.
  • TG downregulated the expression of TGF-β1, Smad2, Smad3, MMP-2, MMP-9, and tissue inhibitor of metalloproteinase-1.
  • TG upregulated the protein expression of Smad7.

Conclusions:

  • Total ginsenoside (TG) exhibits protective effects against bleomycin-induced pulmonary fibrosis.
  • TG's mechanism involves the regulation of the TGF-β1/Smad signaling pathway and the MMP system.
Keywords:
Matrix metalloproteinasePulmonary fibrosisTotal ginsenosideTransforming growth factor β1/Smad signalling pathway

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