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Updated: Jan 26, 2026

Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes
Published on: January 3, 2020
Thermal Destruction of Staphylococcus aureus in Human Milk
F Jane Lin1, Jeffery N Morgan1, Ronald R Eitenmiller1
1Department of Food Science, University of Georgia, Athens, Georgia 30602.
Abstract:
Rates of thermal destruction of Staphylococcus aureus were determined in mature human milk using a continuous flow high-temperature short-time pasteurization system. D and z values for inactiviation of S. aureus were determined from data on survivors capable of forming colonies in an appropriate selective medium. The effects of thermal injury on D and z values were assessed by survivor colony forming units (CFU) on Staphylococcus medium 110 (SMI 10), nutrient agar (NA), Trypticase Soy Agar (TSA), Trypticase Soy Agar with 7.5% NaCi (TSAS) and Baird-Parker medium (BP) (Difco Laboratories, Detroit, MI). D values for inactivation of S. aureus at 52, 58 and 60 and 62°C were used to predict D at 60°C of 15.3 s and 24.3 s when based on survivor CFU in SM 110 and nutrient agar, respectively. The z value was 4.9°C in either medium. D-values for inactivation of S. aureus at 60, 62, 64 and 67°C were used to predict D at 60°C of 41.2 s, 41.0 s and 34.7 s when based on survivor CFU in BP, TSA and TSAS, respectively. The z values were 6.5, 6.5 and 6.4°C, respectively.
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