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Updated: Jan 26, 2026

Loop-Mediated Isothermal Amplification for Screening Salmonella in Animal Food and Confirming Salmonella from Culture Isolation
Published on: May 20, 2020
Dynamics of Salmonella Isolation with Modified Semi-Solid Rappaport-Vassiliadis Medium
Jozef M DE Smedt1, Robert F Bolderdijk1
1Laboratories of Van Houten International, Montezuma-laan 1, B-2410 Herentals, Belgium and Susterfelder-strasse 190, D-5100 Aachen, West Germany.
Abstract:
The effect of low Salmonella cell concentrations in combination with high numbers of competitive flora after pre-enrichment on subsequent isolation with motility enrichment in modified semisolid Rappaport-Vassiliadis (MSRV) was studied. Salmonella was always detected on MSRV if the cell concentration in the preenrichment culture was at least 60 per ml, even if they were outnumbered by competitive bacteria by a factor of 107. In a total of 52 experiments, Salmonella detection on MSRV directly after preenrichment was successful in 46 tests. Combination of direct motility enrichment with additional motility enrichment after 8 h in tetrathionate brilliant green (TBG) broth resulted in 49 positive tests, compared to only 33 positive results after enrichment in TBG for 24 h with subsequent plating on selective agars.
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