Electrosprayed Core⁻Shell Composite Microbeads Based on Pectin-Arabinoxylans for Insulin Carrying: Aggregation and
Agustín Rascón-Chu1, Jonathan A Díaz-Baca2, Elizabeth Carvajal-Millan3
1Research Center for Food and Development, CIAD, A.C., Carretera a La Victoria Km. 0.6, Hermosillo, Sonora 83304, Mexico. arascon@ciad.mx.
Polymers
|April 11, 2019
Summary
This study introduces electrospray fabrication for creating uniform, 1µm composite microbeads from pectin and arabinoxylans. The method prevents aggregation and coalescence, yielding stable core-shell structures for potential drug delivery applications.
Area of Science:
- Materials Science
- Biotechnology
- Chemical Engineering
Background:
- Microparticle and nanoparticle fabrication often suffers from aggregation and polydispersity.
- Controlling particle size and preventing aggregation are critical for biopolymer-based micro/nanomaterials.
Purpose of the Study:
- To evaluate a novel electrospray method for fabricating uniform, low methoxy pectin/arabinoxylans composite microbeads.
- To control particle size and prevent aggregation/coalescence during microbead production.
Main Methods:
- Electrospray fabrication with adjustable voltage, flux, and CaCl₂/ethanol crosslinking solution.
- Utilized light diffraction, scanning electron microscopy (SEM), transmission electron microscopy (TEM), and confocal laser scanning microscopy (CLSM).
- Employed a central composite rotatable design (CCRD) for statistical analysis of variables.
Main Results:
- Achieved stable, spherical core-shell microbeads without aggregation or coalescence when ethanol was included in the crosslinking solution.
- Optimized parameters (11 KV, 75% ethanol, 11% CaCl₂, 0.97 mL/h flow) yielded an average diameter of 1 ± 0.024 μm.
- Identified significant interactions between ethanol/CaCl₂ and flow/voltage influencing particle characteristics.
Conclusions:
- Electrospray is an effective method for producing uniform, aggregation-free composite microbeads.
- The developed method offers precise control over particle size and morphology for biopolymer microparticles.
- This technique shows promise for fabricating well-defined microcarriers for applications like drug delivery.
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