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Synthesis of Soft Polysiloxane-urea Elastomers for Intraocular Lens Application
Published on: March 8, 2019
Preparation of Protein Molecular-Imprinted Polysiloxane Membrane Using Calcium Alginate Film as Matrix and Its
Dong Liu1, Kongyin Zhao2,3, Meng Qi4
1State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, China. techiec@163.com.
Abstract:
Bovine serum albumin (BSA) molecular-imprinted polysiloxane (MIP) membrane was prepared by sol-gel technology, using silanes as the functional monomers, BSA as the template and CaAlg hydrogel film as the matrix. The stress-strain curves of wet CaAlg membrane and molecular-imprinted polysiloxane membrane were investigated. We evaluate the adsorption and recognition properties of MIP membrane. Results showed that the adsorption capacity of BSA-imprinted polysiloxane for BSA reached 28.83 mg/g, which was 2.18 times the non-imprinted polysiloxane (NIP) membrane. The adsorption rate was higher than that of the protein-imprinted hydrogel. BSA-imprinted polysiloxane membrane could identify the protein template from competitive proteins such as bovine hemoglobin, ovalbumin and bovine γ-globulin. In order to obtain the biomaterial that can promote cell adhesion and proliferation, fibronectin (FN)-imprinted polysiloxane (FN-MIP) membrane was obtained by using fibronectin as the template, silanes as functional monomers, and CaAlg hydrogel membrane as the substrate or matrix. The FN-MIP adsorbed more FN than NIP. The FN-imprinted polysiloxane membrane was applied to culture mouse fibroblast cells (L929) and the results proved that the FN-MIP had a better effect on cell adhesion than NIP.
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