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Updated: Jan 26, 2026

Collection and Identification of Pollen from Honey Bee Colonies
Published on: January 19, 2021
Fluorescent Labeling of Polysaccharides from Masson Pine Pollen and Its Effect on RAW264.7 Macrophages
Mengmeng Sun1,2, Fangchen Su3, Jinxin Yang4
1Shandong Provincial Key Laboratory of Animal Resistance Biology, School of Life Science, Shandong Normal University, Jinan 250014, China. sun007_2007@163.com.
Abstract:
In order to explore the immediate effect of polysaccharides and macrophages, polysaccharides from masson pine pollen (PPM60) were labeled with fluorescein isothiocyanate (FITC) by using a chemical-derived method, and the reactant was named PPM60-Tyr-FITC. Direct interaction of PPM60-Tyr-FITC and RAW264.7 macrophages could be detected by flow cytometer (FCM), and this interaction could be inhibited by Pitstop 2 (clathrin inhibitor) and TAK-242 (Toll-like receptor 4 inhibitor). The results of confocal laser scanning microscopy (CLSM) also revealed that there was a co-localization phenomenon between PPM60-Tyr-FITC and RAW264.7 macrophage receptors, and it could be suppressed by Pitstop 2 and TAK-242. It was confirmed that PPM60 enters into RAW264.7 macrophages mainly through endocytosis, rather than the phagocytosis, and TLR4 played a mediating role.
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