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Polyhexamethylene Biguanide and Nadifloxacin Self-Assembled Nanoparticles: Antimicrobial Effects against

Nor Fadhilah Kamaruzzaman1,2, Maria De Fatima Pina3, Alexandru Chivu4

  • 1Department of Pathobiology and Population Science, Royal Veterinary College, University of London, Royal College Street, London NW1 0TU, UK. norfadhilah@umk.edu.my.

Polymers
|April 11, 2019
PubMed

Insights

New nanoparticles effectively treat intracellular MRSA infections. This formulation combines polyhexamethylene biguanide (PHMB) and nadifloxacin, showing reduced toxicity and improved host cell recovery for skin infection treatment.

Area of Science:

  • Pharmacology
  • Nanotechnology
  • Infectious Diseases

Background:

  • Methicillin-resistant Staphylococcus aureus (MRSA) skin infections are challenging due to intracellular bacterial localization.
  • Limited antimicrobial penetration and efficacy within host cells hinder treatment.
  • Novel drug delivery systems are needed to overcome these limitations.

Purpose of the Study:

  • To formulate and evaluate polyhexamethylene biguanide (PHMB)/nadifloxacin nanoparticles for treating intracellular MRSA infections.
  • To assess the efficacy, toxicity, and host cell recovery of the developed nanoparticles.

Main Methods:

  • Formulation of cationic polymer PHMB with topical antibiotic nadifloxacin into nanoparticles.
  • Characterization of nanoparticle size, polydispersity index, zeta potential, and encapsulation efficiency.
  • In vitro testing against intracellular MRSA in infected keratinocytes, assessing bacterial killing, toxicity, and host cell recovery.

Main Results:

  • PHMB/nadifloxacin nanoparticles exhibited a size of 291.3 ± 89.6 nm, PDI of 0.35 ± 0.04, zeta potential of +20.2 ± 4.8 mV, and 58.25 ± 3.4% encapsulation efficiency.
  • The nanoparticles demonstrated significant killing of intracellular MRSA.
  • Compared to free agents, nanoparticles showed reduced toxicity and enhanced host cell recovery.

Conclusions:

  • PHMB/nadifloxacin nanoparticles are effective against intracellular MRSA.
  • This formulation offers a promising therapeutic strategy for skin and soft tissue infections.
  • Further development of these nanoparticles could lead to improved treatment outcomes.
Keywords:
intracellular MRSAnadifloxacinnanoparticlespolyhexamethylene biguanideskin and soft tissue infections

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