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Inositol 1,4,5-Trisphosphate Mediates Nitric-Oxide-Induced Chilling Tolerance and Defense Response in Postharvest
Caifeng Jiao1, Yifeng Chai1, Yuquan Duan1
1Key Opening Laboratory of Agricultural Products Processing and Quality Control, Ministry of Agriculture, Institute of Agro-Food Science and Technology , Chinese Academy of Agricultural Sciences , Beijing 100193 , People's Republic of China.
Abstract:
The function of inositol 1,4,5-trisphosphate (IP3) on nitric oxide (NO)-induced chilling tolerance and defense response in postharvest peach fruit was explored. The postharvest fruit were treated with sodium nitroprusside (SNP, exogenous NO donor), cPTIO (NO scavenger), and neomycin (IP3 inhibitor). It turned out that SNP treatment mitigated chilling injury (CI) and stimulated NO accumulation in postharvest peach fruit. Further, SNP enhanced phosphoinositide-specific phospholipase C (PI-PLC) activity and, thereby, stimulated IP3 prodution. SNP also upregulated the activity and expression of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), glutathione S-transferase (GST), and glutathione reductase (GR). In addition, SNP enhanced the expression of small ubiquitin-like modifier (SUMO) and methionine sulfoxide reductase (MSR) and weakened the activity and expression of lipoxygenase (LOX) and phospholipase D (PLD). These above impacts stimulated by SNP treatment were blocked by the addition of cPTIO and neomycin. Overall, IP3 was involved in NO-enhanced chilling tolerance and defense response in postharvest peach fruit.
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