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Detergent Purification of Membrane Proteins

Detergent Purification of Membrane Proteins

Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...

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Related Experiment Video

Updated: Sep 30, 2025

Proof-of-Concept for Gas-Entrapping Membranes Derived from Water-Loving SiO2/Si/SiO2 Wafers for Green Desalination

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Bio-Inspired Polymer Membrane Surface Cleaning.

Agnes Schulze¹, Daniel Breite², Yongkyum Kim³

¹Leibniz Institute of Surface Modification, Permoserstr 15, D-04318 Leipzig, Germany. Agnes.Schulze@iom-leipzig.de.

|April 12, 2019

Summary

This summary is machine-generated.

This study developed self-cleaning polyethersulfone membranes by immobilizing pancreatin (a digestive enzyme mixture). These biocatalytically active membranes can degrade fouling layers, restoring permeability by adjusting pH and temperature.

Keywords:

catalytic properties enzyme immobilization polymer membrane self-cleaning surface modification

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Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

Published on: April 7, 2017

Area of Science:

Biocatalysis
Membrane Science
Surface Chemistry

Background:

Polyethersulfone (PES) membranes are widely used but susceptible to fouling, reducing performance.
Developing self-cleaning membranes is crucial for sustainable water treatment and industrial processes.
Enzyme immobilization offers a promising route to impart biocatalytic activity to membrane surfaces.

Purpose of the Study:

To create polyethersulfone membranes with a biocatalytically active surface.
To investigate the self-cleaning capabilities of enzyme-modified membranes.
To characterize the properties and performance of the modified membranes.

Main Methods:

Covalent immobilization of pancreatin (protease, lipase, amylase) onto polyethersulfone membranes.
Characterization using enzyme activity assays, porosimetry, X-ray photoelectron spectroscopy (XPS), and scanning electron microscopy (SEM).
Fouling tests using protein and oil solutions, followed by assessment of self-cleaning performance via permeability recovery.

Main Results:

Successful covalent immobilization of pancreatin, conferring biocatalytic activity.
Demonstrated self-cleaning properties: membranes degraded fouling layers upon pH and temperature adjustment.
Regained initial water permeation flux after fouling and self-cleaning cycles.
Characterization confirmed enzyme presence and surface modification.

Conclusions:

Pancreatin-immobilized polyethersulfone membranes exhibit effective self-cleaning properties.
Enzyme activity can be activated by environmental stimuli (pH, temperature) to degrade foulants.
This approach offers a sustainable solution for maintaining membrane performance and extending operational life.