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Measuring Proliferation of Vascular Smooth Muscle Cells Using Click Chemistry

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Published on: October 30, 2019

Long noncoding RNA LINC00341 promotes the vascular smooth muscle cells proliferation and migration via miR-214/FOXO4

Xiu Liu¹, Bing-Dong Ma², Shan Liu³

¹Department of Cardiology, Yantai Affiliated Hospital of Binzhou Medical University Yantai, Shandong, China.

American Journal of Translational Research

|April 12, 2019

Abstract:

Increasing evidences have indicated the vital roles of long noncoding RNA (lncRNA) in the atherosclerosis. However, whether lncRNA LINC00341 play pivotal roles in the vascular smooth muscle cells (VSMCs) is still unclear. This work presents the authentic functions of LINC00341 on the proliferation and migration of VSMCs and unveils the underlying mechanism. Functional experiment data demonstrated that LINC00341 expression was increased in the ox-LDL induced VSMCs with dose-dependent and time-dependent mode. Moreover, the knockdown of LINC00341 suppressed the proliferation and migration ability of VSMCs. Mechanically, we found that LINC00341 promoted the FOXO4 protein expression via sponging miR-214, which, in return, resulting in the transcription activation of LINC00341. In conclusion, the results conclude that LINC00341 promotes the proliferation and migration of VSMCs and confirm the positive feedback loop of LINC00341/miR-214/FOXO4 axis.

Keywords:

FOXO4 LINC00341 Vascular smooth muscle cells miR-214 migration proliferation

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