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Altered expression patterns of complement factor H and miR-146a genes in acute-chronic phases in experimental

Saba Gharibi1, Bahram Moghimi2, Dariush Haghmorad3,4

  • 1Department of Genetics, Faculty of Medicine, International Campus, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.

Journal of Cellular Physiology
|April 12, 2019
PubMed

Insights

Complement factor H (CFH) and miR-146a gene expression varied in an experimental autoimmune encephalomyelitis (EAE) mouse model. These findings suggest potential roles for CFH and miR-146a as biomarkers in multiple sclerosis (MS) disease progression.

Area of Science:

  • * Neuroimmunology and molecular biology.
  • * Investigating immune system components in central nervous system disorders.

Background:

  • * Growing evidence links the complement pathway to multiple sclerosis (MS) pathogenesis.
  • * MicroRNAs (miRNAs) are crucial in modulating immune responses within the central nervous system.

Purpose of the Study:

  • * To examine the expression profiles of complement factor H (CFH) and miR-146a in a mouse model of MS.
  • * To assess the potential of CFH and miR-146a as biomarkers for MS disease status.

Main Methods:

  • * Real-time polymerase chain reaction was used to measure gene expression.
  • * CFH and miR-146a expression levels were analyzed in liver and brain tissues during acute and chronic phases of experimental autoimmune encephalomyelitis (EAE).
  • * Comparisons were made between EAE mice and healthy controls.

Main Results:

  • * Liver CFH gene expression increased in the chronic EAE phase compared to the acute phase.
  • * Liver miR-146a expression significantly decreased in the chronic EAE group compared to acute and control groups.
  • * Brain CFH gene expression significantly decreased in both acute and chronic EAE phases compared to healthy mice.

Conclusions:

  • * The complement system and miR-146a show probable involvement in immune-related diseases like MS.
  • * These findings offer insights into the pathogenesis of MS.
  • * Further research is needed to validate CFH protein levels and miR-146a targets in human MS patient samples (serum and cerebrospinal fluid).
Keywords:
EAEmiR-146amicroRNAneuroinflammationrelapsing-remitting multiple sclerosis

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