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Testis-specific calcium-binding protein CBP86-IV (CABYR) binds with phosphoglycerate kinase 2 in vitro and in vivo

Shulin Shen1, Dongrun Li1, Jihong Liang1

  • 1Department of Andrology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.

Andrologia
|April 12, 2019
PubMed

Insights

Researchers identified proteins interacting with testis-specific calcium-binding protein CBP86-IV (CABYR) in human sperm. Phosphoglycerate kinase 2 (PGK2) was confirmed to interact with CABYR, advancing our understanding of sperm function.

Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Proteomics

Background:

  • Testis-specific calcium-binding protein CBP86-IV (CABYR) plays a role in human spermatozoa.
  • Understanding protein interactions is crucial for elucidating CABYR function in sperm.

Purpose of the Study:

  • To identify proteins that interact with CABYR in human spermatozoa.
  • To confirm the interaction between CABYR and identified proteins using multiple experimental approaches.

Main Methods:

  • Recombinant expression of CABYR in Escherichia coli.
  • Co-immunoprecipitation (Co-IP) assays in E. coli and human sperm lysates.
  • Mass spectrometry for protein identification.
  • Yeast two-hybrid system for interaction confirmation.

Main Results:

  • Thirteen potential interacting proteins were identified in E. coli lysate.
  • Phosphoglycerate kinase 2 (PGK2) showed positive interaction results in both reverse Co-IP and yeast two-hybrid assays.
  • The interaction between CABYR and PGK2 was confirmed in human sperm cells.

Conclusions:

  • CABYR interacts with PGK2 in human sperm.
  • This interaction may be significant for sperm function and male fertility.
  • Further research into CABYR-PGK2 complex functions is warranted.
Keywords:
phosphoglycerate kinase 2protein interactionspermatozoatestis-specific calcium-binding protein CBP86-IV

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