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Related Experiment Video

Updated: Jan 26, 2026

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Published on: July 16, 2019

DNA-Based Photoacoustic Nanosensor for Interferon Gamma Detection.

Jennifer Morales¹, Robert H Pawle², Namik Akkilic³

¹Department of Bioengineering , Northeastern University , Boston , Massachusetts 02115 , United States.

|April 12, 2019

Summary

Researchers developed a novel DNA nanosensor for detecting interferon gamma using photoacoustics. This tool offers real-time protein monitoring for improved medical diagnostics and disease research.

Keywords:

DNA cytokine interferon-gamma nano photoacoustic phthalocyanine sensor

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Last Updated: Jan 26, 2026

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Published on: July 16, 2019

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Published on: July 16, 2018

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Published on: January 20, 2019

Area of Science:

Biomedical Engineering
Molecular Biology
Nanotechnology

Background:

Protein level monitoring is crucial for understanding physiological roles, homeostasis, and diseases.
Interferon gamma is a clinically relevant protein with key immunoregulatory functions.
Continuous in vivo protein monitoring tools are needed for real-time patient assessment.

Purpose of the Study:

To develop a DNA-based nanosensor for the photoacoustic detection of interferon gamma.
To create a proof-of-concept sensor for in vivo protein tracking.
To adapt the technology for detecting other proteins of interest.

Main Methods:

Development of a DNA nanosensor incorporating receptors and a phthalocyanine dye.
Utilizing photoacoustic detection for signal generation.
Surface plasmon resonance kinetic analysis to assess sensor performance.

Main Results:

The nanosensor demonstrated responsiveness and reversibility to interferon gamma with nanomolar affinity (K_D1 = 167 nM, K_D2 = 316 nM).
A novel phthalocyanine dye, when J-aggregated, increased the photoacoustic signal by 22.5%.
DNA structure bending upon receptor binding induced dye stacking, increasing the photoacoustic signal by 55% in the presence of 10 μM interferon gamma.

Conclusions:

A novel DNA-based photoacoustic nanosensor for interferon gamma detection was successfully developed.
The sensor exhibits high sensitivity and specificity, with potential for adaptation to other protein targets.
This technology represents a significant advancement for in vivo protein monitoring in research and medicine.