Mangrove Fishes Rely on Emersion Behavior and Physiological Tolerance to Persist in Sulfidic Environments

Hydrogen sulfide (H ₂ S) is a potent respiratory toxin that makes sulfidic environments tolerable to only a few organisms. We report the presence of fishes ( Kryptolebias marmoratus , Poecilia orri , Gambusia sp., and Dormitator maculatus ) in Belizean mangrove pools with extremely high H ₂ S concentrations (up to 1,166 μM) that would be lethal for most fishes. Thus, we asked whether the three most prevalent species ( Kryptolebias , Poecilia , and Gambusia ) persist in sulfidic pools because they are exceptionally H ₂ S tolerant and/or because they can leave water (emerse) and completely avoid H ₂ S. We show that both physiological tolerance and emersion behavior are important. Kryptolebias demonstrated high H ₂ S tolerance, as they lost equilibrium significantly later than Poecilia and Gambusia during H ₂ S exposure ( $$ μM H ₂ S). However, the fact that all species lost equilibrium at an ecologically relevant [H ₂ S] suggests that physiological tolerance may suffice at moderate H ₂ S concentrations but that another strategy is required to endure higher concentrations. In support of the avoidance behavior hypothesis, H ₂ S elicited an emersion response in all species. Kryptolebias was most sensitive to H ₂ S and emersed at H ₂ S concentrations 52% and 34% lower than Poecilia and Gambusia , respectively. Moreover, H ₂ S exposure caused Kryptolebias to emerse more frequently and spend more time out of water compared to control conditions. We suggest that physiological H ₂ S tolerance and emersion behavior are complementary strategies. The superior H ₂ S tolerance and amphibious capability of Kryptolebias may explain why this species was more prevalent in H ₂ S-rich environments than other local fishes.