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Related Experiment Video

Updated: Jan 26, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR

Published on: November 4, 2016

Sub-inhibitory membrane damage undermines Staphylococcus aureus virulence.

Ayelet Sarah Hershkovits¹, Igor Pozdnyakov¹, Ohad Meir¹

¹Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel.

Biochimica Et Biophysica Acta. Biomembranes

|April 12, 2019

Summary

This summary is machine-generated.

The lipopeptide C10OOc12O enhances plasma antibacterial activity and sensitizes MRSA to oxacillin by disrupting bacterial quorum sensing. This suggests potential for new anti-virulence therapies against Gram-positive bacteria.

Keywords:

Antibiotic resistance Innate immunity Mechanisms of action Peptidomimetics Proton motive force Synergism of action

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Area of Science:

Microbiology
Biochemistry
Pharmacology

Background:

Gram-positive bacteria (GPB) pose significant health threats.
Novel antibacterial agents are crucial for combating resistance.
Membrane-active lipopeptides offer a promising therapeutic avenue.

Purpose of the Study:

To investigate the antibacterial properties of the lipopeptide C10OOc12O against GPB.
To evaluate its synergistic effects with antibiotics and its impact on bacterial virulence.
To elucidate the mechanism of action of C10OOc12O.

Main Methods:

Minimal inhibitory concentration (MIC) and kinetic assays in culture media and plasma.
Checkerboard assay for chemo-sensitization.
Membrane potential, ATP levels, and enzyme activity assays.
Assessment of quorum sensing-regulated virulence factors.

Main Results:

C10OOc12O showed enhanced activity in human plasma compared to culture media.
Sub-inhibitory concentrations damaged bacterial membranes, reduced transmembrane potential, and transiently affected ATP levels.
C10OOc12O inhibited key virulence factors (lipase, α-toxin) and resensitized methicillin-resistant Staphylococcus aureus (MRSA) USA300 to oxacillin.
The lipopeptide affected the quorum sensing regulator, weakening bacterial defenses.

Conclusions:

C10OOc12O exhibits potent anti-virulence activity against S. aureus by targeting quorum sensing.
Its ability to potentiate antibiotic activity and disrupt virulence factors highlights its therapeutic potential.
C10OOc12O represents a promising candidate for novel anti-virulence strategies against GPB infections.