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Electrophoretic Separation of Proteins
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Electrophoretic Separation on an Origami Paper-Based Analytical Device Using a Portable Power Bank
Yu Matsuda1, Katsunori Sakai2, Hiroki Yamaguchi3
1Department of Modern Mechanical Engineering, Waseda University, 3-4-1 Ookubo, Shinjuku-ku, Tokyo, 169-8555, Japan. y.matsuda@aoni.waseda.jp.
Sensors (Basel, Switzerland)
|April 13, 2019
Summary
This study introduces a novel origami paper-based analytical device (PAD) for rapid electrophoretic separation of amino acids. The device achieves quick separation within 4 minutes, overcoming pH variation challenges in paper electrophoresis.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Electrophoresis of ampholytes like amino acids on paper is challenging due to temporal pH variations.
- Existing paper-based methods struggle with unstable pH gradients, limiting separation efficiency.
Purpose of the Study:
- To develop a stable and efficient paper-based analytical device (PAD) for ampholyte electrophoresis.
- To overcome the limitations of pH variation in time during paper electrophoresis.
Main Methods:
- Designed and fabricated a paper-based analytical device (PAD) utilizing an origami structure.
- Employed a low operating voltage (5 V) powered by a portable power bank.
- Demonstrated electrophoretic separation of pI markers (pI 5.5 and 8.7).
Main Results:
- Achieved electrophoretic separation of pI markers within 4 minutes, before significant pH variation occurred.
- Demonstrated that the separation distance can be increased by modifying the origami structure.
- Confirmed the utility of the proposed PAD for effective electrophoretic separation on paper.
Conclusions:
- The origami-structured PAD offers a viable solution for rapid and stable electrophoretic separation of ampholytes.
- This low-voltage, paper-based system shows promise for portable analytical applications.
- Further optimization of the origami design can enhance separation performance.
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