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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador
J E Villacís1,2, M Bovera3, D Romero-Alvarez4
1Carrera de Bioquímica Clínica, Facultad de Medicina, Pontificia Universidad Católica del Ecuador, Quito, Ecuador.
This study reports the first detection of Acinetobacter baumannii sequence type 32 carrying the New Delhi metallo-β-lactamase gene in Ecuador. This finding highlights the emergence of carbapenem resistance in this region.
Area of Science:
- Microbiology
- Genetics
- Infectious Diseases
Background:
- Acinetobacter baumannii is a significant opportunistic pathogen.
- Carbapenem resistance is a growing global health concern.
- Metallo-β-lactamases (MBLs) confer resistance to carbapenems.
Purpose of the Study:
- To document a clinical case of Acinetobacter baumannii ST32 in Ecuador.
- To identify the presence of New Delhi metallo-β-lactamase (NDM) in this isolate.
Main Methods:
- Multilocus sequence typing (MLST) for species and ST identification.
- Synergy testing (imipenem-EDTA disc) and carbapenem inactivation method (CIM) for carbapenemase detection.
- PCR amplification and sequencing to confirm the carbapenemase gene (blaNDM-1).
Main Results:
- Acinetobacter baumannii ST32 was identified.
- The blaNDM-1 gene was detected in the ST32 isolate.
- The blaNDM-1 gene was isolated from a plasmid via PCR.
Conclusions:
- This is the first report of Acinetobacter baumannii ST32 carrying the blaNDM-1 gene in Ecuador.
- This finding signifies the emergence of a concerning multidrug-resistant strain in the region.
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