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The application value of ultra-short echo time MRI in the quantification of liver iron overload in a rat model
Qiaoling Wu1, Xiuwei Fu2, Zhizheng Zhuo3
1Tianjin University of Traditional Chinese Medicine, Tianjin 300192, China.
Background:
The quantitative evaluation of liver iron concentration (LIC) is important in guiding the treatment of blood transfusion-dependent patients. Conventionally, LIC is assessed through R2*or R2 values using magnetic resonance imaging (MRI). However, most of the studies using MRI to determine iron overload were restricted by the minimum echo time, so that severe iron overload could hardly be quantified. In our study, we demonstrate a new approach to overcome the limitation of the shortest echo time using ultra-short echo time (UTE) MRI to quantify liver iron overload of varying degrees in a rat model.
Methods:
Sixty female Sprague-Dawley rats were included and randomly assigned into 10 equal groups. Group 1 was not injected with iron dextran. Groups 2 to 10 were intraperitoneally injected with iron dextran at a dose of 15 mg/kg every 3 days. On every 6th day, one group was randomly selected from groups 2 to 10 for MRI scanning and liver iron concentration (LIC) detection. For groups 1 to 10, images were acquired by UTE sequence using a 3.0T MR scanner, and the T2* value and R2* value were obtained (R2* =1/T2*). In addition, LIC was measured using an atomic absorption photometer. The correlation analysis between R2* value and LIC was performed and the regression equation of R2* and LIC was established and its reliability verified.
Results:
For groups 1 to 10, R2* values and LIC ranged from 60.16±4.76 to 1,306.90±42.26 Hz and from 0.84±0.11 to 5.89±2.64 mg/g dry, respectively. The R2* value was linearly correlated to the LIC (r=0.897, P<0.001), and the linear regression equation was LIC = 0.005 × R2* + 1.783. The validation analysis results showed that the intragroup correlation coefficient (ICC) between the predicted and measured LIC was 89.5%.
Conclusions:
The UTE sequence could be used for quantification of varying degrees of hepatic iron overload in the rat model, and the LIC could be predicted by using the R2* value on an MR 3.0T scanner.
Insights
Ultra-short echo time (UTE) MRI effectively quantifies liver iron concentration (LIC) in rats, overcoming limitations of conventional MRI. This method accurately predicts iron overload using R2* values, aiding treatment guidance.
Area of Science:
- Biomedical Imaging
- Magnetic Resonance Imaging
- Medical Diagnostics
Background:
- Accurate quantification of liver iron concentration (LIC) is crucial for managing transfusion-dependent patients.
- Conventional MRI methods (R2*, R2) for LIC assessment are limited by minimum echo time, hindering quantification of severe iron overload.
Purpose of the Study:
- To develop and validate an ultra-short echo time (UTE) MRI approach for quantifying varying degrees of liver iron overload.
- To overcome the limitations of minimum echo time in conventional MRI for iron overload assessment.
Main Methods:
- UTE MRI sequence on a 3.0T scanner was used in a rat model (n=60) with induced iron overload.
- R2* values were obtained and correlated with liver iron concentration (LIC) measured by atomic absorption spectrophotometry.
- Regression analysis established a predictive equation for LIC based on R2* values.
Main Results:
- A strong linear correlation was found between R2* values and LIC (r=0.897, P<0.001).
- The established regression equation was LIC = 0.005 × R2* + 1.783.
- Validation analysis showed high agreement between predicted and measured LIC (ICC=89.5%).
Conclusions:
- UTE MRI is a viable method for quantifying diverse levels of hepatic iron overload in a rat model.
- R2* values derived from UTE MRI on a 3.0T scanner can reliably predict LIC.
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