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Data on draft genome sequence of Bacillus sp. strain VKPM B-3276 isolated from Culex pipiens larvae

V V Zinina1, A A Korzhenkov1, A V Tepliuk1

  • 1National Research Center "Kurchatov Institute", Moscow, 123182, Russia.

Data in Brief
|April 13, 2019
PubMed

Insights

The draft genome of Bacillus sp. VKPM B-3276, isolated from mosquito larvae, reveals genes for decomposing chitin. This bacterium is a promising candidate for developing new entomopathogenic agents.

Area of Science:

  • Microbiology
  • Genomics
  • Biotechnology

Background:

  • Bacillus sp. VKPM B-3276 is a gram-positive bacterium isolated from dead Culex pipiens larvae.
  • This strain is preserved in the Russian National Collection of Industrial Microorganisms.
  • It is being considered for the development of novel entomopathogenic agents.

Purpose of the Study:

  • To present the draft genome sequence of Bacillus sp. VKPM B-3276.
  • To identify genes related to potential entomopathogenic activity, particularly those involved in the decomposition of insect cuticle components.

Main Methods:

  • Whole-genome sequencing was performed on Bacillus sp. VKPM B-3276.
  • Bioinformatic analysis using RAST/SEED was employed to annotate the genome.
  • Gene content was analyzed, focusing on functional categories and enzyme-coding genes.

Main Results:

  • The draft genome sequence of Bacillus sp. VKPM B-3276 has a size of 6,126,346 bp with 99.43% completeness.
  • The genome contains 6518 protein-coding sequences and 111 RNAs genes.
  • Approximately 13% of protein-coding genes (271) fall under the 'Carbohydrates' category, including around 50 enzymes involved in the decomposition of chitin and peptidoglycan.

Conclusions:

  • The genome analysis confirms the presence of enzymes crucial for breaking down chitin and related molecules, supporting its potential as an entomopathogen.
  • The genomic data provides a foundation for further research and development of Bacillus sp. VKPM B-3276 as a biocontrol agent against insect pests.
  • The draft genome sequence has been deposited in public databases (DBJ/EMBL/GenBank) under accession numbers RZHM00000000, PRJNA511803, and SAMN10644103.
Keywords:
BacillusDe novo assemblyDraft genome assemblyEntomopathogenic bacteriaMosquito biocontrol

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