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Updated: Jan 26, 2026

Dissection of Xenopus laevis Neural Crest for in vitro Explant Culture or in vivo Transplantation
Published on: March 4, 2014
Deciphering the Neural Crest Contribution to Cephalic Development with Avian Embryos.
Moussab Alrajeh1, Zuzana Vavrusova1,2, Sophie E Creuzet3
1Institut des Neurosciences Paris-Saclay, Neuro-PSI CNRS UMR 9197, Gif-sur-Yvette, France.
The quail-chick system is a powerful tool for studying cell development and migration. It enables detailed analysis of neural crest cells and craniofacial morphogenesis, advancing our understanding of developmental biology.
Area of Science:
- Developmental Biology
- Cell Biology
- Genomics
Background:
- The quail-chick chimera system has been instrumental in tracking cell lineages during development.
- It has significantly advanced the understanding of neural crest cell biology and craniofacial morphogenesis.
- This model is crucial for studying cell induction, migration, and interactions.
Purpose of the Study:
- To highlight the utility of the quail-chick system in developmental and cell biology research.
- To emphasize its role in functional genomics and understanding cell differentiation.
- To showcase its potential for deciphering molecular codes in tissue interactions.
Main Methods:
- Utilizing quail-chick chimeras for cell tracking and lineage analysis.
- Employing in ovo electroporation for functional genomics studies.
- Combining tissue manipulation with gene expression analysis.
Main Results:
- The system allows for precise tracking of cells during complex morphogenetic movements and migrations.
- It has been pivotal in understanding neural crest development and its contribution to craniofacial structures.
- Enables simultaneous tissue and gene manipulation within the same cell population.
Conclusions:
- The quail-chick system offers unparalleled spatiotemporal accuracy for studying cell positioning and differentiation.
- It is a versatile model for dissecting instructive versus permissive tissue interactions.
- This system continues to be a resourceful platform for functional studies in developmental biology.
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09:33Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
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