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Updated: Jan 26, 2026

Culturing and Manipulation of O9-1 Neural Crest Cells
Published on: October 9, 2018
Live Imaging of Neural Crest and Pigment Cells and Transient Transgenic Manipulation of Gene Activity
Reyna Deeya Ballim1, Yusuke Nagao1, Robert Neil Kelsh2
1Department of Biology and Biochemistry, University of Bath, Bath, UK.
Abstract:
Neural crest cells are a highly multipotent and migratory cell type that are important for adult pigment pattern formation, cellular homeostasis, and regeneration. The optical transparency and accessibility of fish embryos makes them particularly well-suited to high-resolution analysis of neural crest development. However, the dispersive nature of these cells adds to the challenge of their study. We describe key protocols for the analysis of neural crest development in zebrafish and medaka, including live imaging of neural crest cells and differentiating pigment cells and transient transgenesis assays that can be used to manipulate neural crest development.
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