Localization of AMPA, kainate, and NMDA receptor mRNAs in the pigeon cerebellum

In the present study, we investigated the location of mRNAs for three types of ionotropic glutamate receptors (iGluRs) in the pigeon cerebellum and then compared the results with those of mammals. The following nine iGluRs subunits were analyzed by in situ hybridization: AMPA receptors (GluA1, GluA2, GluA3, and GluA4), kainate receptors (GluK1, GluK2, and GluK4), and NMDA receptors (GluN1 and GluN2A). Subunit hybridization revealed expression in different cell types of the cerebellar cortex: Purkinje cells expressed most subunits, including AMPA receptors (GluA1, GluA2, and GluA3), kainate receptors (GluK1 and GluK4), and NMDA receptors (GluN1); granule cells expressed four subunits of kainate (GluK1 and GluK2) and NMDA receptors (GluN1 and GluN2A); stellate and basket cells expressed GluK1, GluK2, and GluN1; Golgi cells expressed GluA1, GluA3, and GluN1; and Bergmann glial cells expressed only AMPA receptors (GluA2 and GluA4). Cerebellar nuclei showed no AMPA subunit signals, whereas kainate and NMDA receptors were observed in the five cerebellar nuclei divisions (CbL, CbMic, CbMim, CbMin, and CbMvm). The five divisions showed weak expression of GluK1, GluK2, and GluN2A; moderate to intense expression of GluK4; and intense expression of GluN1. These results demonstrate that in pigeons the cerebellar cortex expresses AMPA, kainate, and NMDA receptors, while the cerebellar nuclei express kainate and NMDA receptors. Taken together, these findings provide anatomical data for further analysis of the functions of iGluR-expressing neurons in glutamatergic circuits of the avian cerebellum.