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Enhanced Recovery of Plesiomonas shigelloides following an Enrichment Technique 1
Susan M Freund1, John A Koburger1, Cheng-I Wei1
1Food Science and Human Nutrition Department, University of Florida, Gainesville, Florida 32611.
Abstract:
Enrichment techniques using five broths (gram-negative broth, alkaline peptone water, tetrathionate broth without iodine and two Plesiomonas broths) were compared to direct plating methods using freshwater samples to determine their ability to increase the isolation rate of Plesiomonas shigelloides , a suspected food and waterbome pathogen. Tetrathionate broth consistently gave significantly (p<0.05) greater recovery of P. shigelloides than the other four broths tested as well as by direct plating. Incubation of the enrichment broths at 40°C also resulted in significantly higher recovery of Plesiomonas than at 35°C. It is therefore suggested that for routine monitoring of P. shigelloides , tetrathionate broth incubated at 40°C be used for enrichment before plating.
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SN1 Reaction: Stereochemistry
In the first step of an SN1 reaction, the bond between the electrophilic carbon and the leaving group ionizes to generate the carbocation intermediate. The second step of the mechanism is the nucleophilic attack.
In the formed carbocation, the positively charged carbon is sp2 hybridized with a trigonal planar geometry. As all the three substituents lie on the same plane, a plane of symmetry for the...

