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Updated: Jan 26, 2026

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages
Published on: October 25, 2017
Millipore Filtration and Use of RV Medium for Isolation of Salmonella from Preenrichment Broths
R B Truscott1, Anna M Lammerding1
1Agriculture Canada, Animal Pathology Laboratory, 110 Stone Road W., Guelph, Ontario N1G 3W4, Canada.
Abstract:
A filtration procedure for isolation of Salmonella from preen-richment broth is described. Five ml of broth are passed through a Millipore AP15 pre-filter above a .45 μm Millipore filter. The filter is transferred to 25 ml of Rappaport-Vassiliadis broth and incubated at 42°C. Of 76 naturally and 55 artificially contaminated meat and poultry samples, 102 were positive for Salmonella . Of these, 99 were isolated using the filtration technique, 93% of which were obtained following a 6-h incubation period. Isolations from tetrathionate brilliant green and selenite cystine broth were 93 and 84, respectively.
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