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A Facile and Eco-friendly Route to Fabricate PolyLactic Acid Scaffolds with Graded Pore Size
Published on: October 17, 2016
Application of Chitinous Materials in Production and Purification of a Poly(l-lactic acid) Depolymerase from
Tzu-Wen Liang1,2, Shan-Ni Jen3, Anh Dzung Nguyen4
1Department of Chemistry, Tamkang University, New Taipei City 25137, Taiwan. ltw27@ms55.hinet.net.
Abstract:
The management of fishery residues and plastics is considered to be a vital strategy for conserving resources and maintaining the quality of the environment. Poly(l-lactic acid) (PLA) is a commercially promising, renewable, and biodegradable plastic. In this study, a PLA depolymerase was produced in a squid pen powder (SPP) and recycled plastic waste (PLA powder)-containing medium by Pseudomonas tamsuii TKU015, a bacterial strain isolated from Taiwanese soil. This PLA depolymerase had a molecular weight of 58 kDa and was purified to homogeneity from the supernatant of a TKU015 culture. The optimum pH of TKU015 PLA depolymerase is 10, and the optimal temperature of the enzyme is 60 °C. In addition to PLA, TKU015 PLA depolymerase degraded fibrinogen and tributyrin, but did not hydrolyze casein, triolein, and poly(β-hydroxybutyrate). Taken together, these data demonstrate that P. tamsuii TKU015 produces a PLA depolymerase to utilize SPP and polylactide as carbon/nitrogen sources.
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