Related Experiment Video
Updated: Jan 26, 2026
08:12
Killer Artificial Antigen Presenting Cells KaAPC for Efficient In Vitro Depletion of Human Antigen-specific T Cells
Published on: August 11, 2014
10.0K
Fluorescent BAPAD Dendrimeric Antigens Are Efficiently Internalized by Human Dendritic Cells
Pablo Mesa-Antunez1,2, Daniel Collado3,4, Yolanda Vida5,6
1Department of Organic Chemistry, University of Malaga, IBIMA, 29071 Malaga, Spain. pmantunez@uma.es.
Polymers
|April 14, 2019
Summary
Researchers developed a novel fluorescent dendrimeric antigen (DeAn) using all-aliphatic polyamide dendrimers (BAPAD). This synthetic antigen shows potential for amoxicillin delivery and drug internalization by dendritic cells.
Area of Science:
- Polymer Chemistry
- Nanotechnology
- Immunology
Background:
- Dendrimers offer unique properties for drug delivery.
- Amoxicillin allergy affects many patients.
- Targeted drug delivery to dendritic cells is a key area in immunology.
Purpose of the Study:
- To synthesize a novel fluorescent dendrimeric antigen (DeAn) using a new class of dendrimers.
- To evaluate the potential of DeAn as a carrier for amoxicillin.
- To assess the internalization and localization of DeAn by dendritic cells.
Main Methods:
- Synthesis of all-aliphatic polyamide dendrimers (BAPAD) with amoxicilloyl terminal groups.
- Characterization of fluorescence properties using PBS and fluorescence microscopy.
- Biomedical assay using dendritic cells from allergic and tolerant patients.
Main Results:
- Successful synthesis of fluorescent DeAn with high quantum yields.
- DeAn demonstrated non-toxicity and efficient internalization by dendritic cells.
- Confocal microscopy revealed preferential cytoplasmic accumulation of DeAn.
Conclusions:
- BAPAD dendrimers are suitable scaffolds for developing synthetic antigens.
- DeAn shows promise as a carrier for amoxicillin drug internalization.
- These findings support the potential of BAPAD dendrons in biomedical applications.
Related Concept Videos
01:05Total Internal Reflection Fluorescence Microscopy
11.1K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
11.1K
01:22Antigen Presenting Cells
3.1K
The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
3.1K
01:31Internal Receptors
74.4K
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.4K
02:00Internal Energy
36.6K
The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
36.6K
01:29Internal Energy
6.9K
The internal energy of a thermodynamic system is the sum of the kinetic and potential energies of all the molecules or entities in the system. The kinetic energy of an individual molecule includes contributions due to its rotation and vibration, as well as its translational energy. The potential energy is associated only with the interactions between one molecule and the other molecules of the system. Neither the system's location nor its motion is of any consequence as far as the internal...
6.9K
01:23Neurons: The Cell Body and the Dendrites
6.8K
A typical nerve cell comprises three main components: the cell body, dendrites, and the axon. The cell body, also known as the soma or perikaryon, serves as the central biosynthetic hub housing a nucleus surrounded by cytoplasm containing organelles commonly found in most cells. Notably, Nissl bodies, clusters of the rough endoplasmic reticulum and free ribosomes responsible for protein synthesis, are distinctive features of the neuronal cell body. As neurons age, aggregates of a brown pigment...
6.8K