Related Experiment Video

Updated: Jan 26, 2026

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
08:36

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Published on: May 26, 2018

12.2K

Reference levels for glucose-6-phosphate dehydrogenase enzyme activity in infants 7-90 days old in Taiwan

Wen-Chien Yang1, Shin Tai2, Chu-Ling Hsu3

  • 1Department of Pediatrics, National Taiwan University Hospital Hsin-Chu Branch, Hsinchu, Taiwan; Department of Global Health and Population, Harvard T. H. Chan School of Public Health, Boston, United States.

Journal of the Formosan Medical Association = Taiwan Yi Zhi
|April 14, 2019
PubMed

Insights

Newborn screening for glucose-6-phosphate dehydrogenase (G6PD) deficiency in Taiwan now has reference values for infants aged 7-90 days. This study determined G6PD enzyme activity levels across different genotypes to aid accurate diagnosis.

Area of Science:

  • Biochemistry
  • Genetics
  • Pediatrics

Background:

  • Taiwan has implemented nationwide newborn screening for glucose-6-phosphate dehydrogenase (G6PD) deficiency since 1987.
  • G6PD enzyme activity levels are used for diagnosis confirmation.
  • Reference values for G6PD enzyme activity were unavailable for infants aged 7-90 days.

Purpose of the Study:

  • To determine glucose-6-phosphate dehydrogenase (G6PD) enzyme activity levels in infants aged 7-90 days.
  • To establish reference values for G6PD enzyme activity based on different genotypes.
  • To support accurate diagnosis of G6PD deficiency in infants.

Main Methods:

  • Enrolled 410 term infants aged 7-90 days at National Taiwan University Hospital Hsinchu branch between January 2016 and June 2017.
  • Compared G6PD enzyme activities among different genotype groups.
  • Analyzed G6PD enzyme activity levels in relation to infant age and genotype.

Main Results:

  • G6PD enzyme activity was negatively correlated with age (R = -0.212, p = 0.01).
  • Significant differences in G6PD enzyme activity were observed among infants with different G6PD genotypes (hemizygotes, heterozygotes, and non-mutated).
  • Specific G6PD enzyme activity levels were reported for infants under and over 30 days old across genotypes.

Conclusions:

  • Established G6PD enzyme activity levels for infants aged 7-90 days in Taiwan.
  • Highlighted the importance of genotype-specific reference data for accurate G6PD deficiency diagnosis.
  • Emphasized the need for defined cutoff values to aid pediatricians in diagnosing G6PD deficiency.
Abstract

Keywords:
G6PDInfantReference levelTaiwan

More Related Videos

Experience is Instrumental in Tuning a Link Between Language and Cognition: Evidence from 6- to 7- Month-Old Infants' Object Categorization05:35

Experience is Instrumental in Tuning a Link Between Language and Cognition: Evidence from 6- to 7- Month-Old Infants' Object Categorization

Published on: April 19, 2017

7.1K
Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice08:43

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

Published on: January 31, 2020

7.3K

Related Concept Videos

Enzymes and Activation Energy01:13

Enzymes and Activation Energy

The activation energy (or free energy of activation), abbreviated as Ea, is the small amount of energy input necessary for all chemical reactions to occur. During chemical reactions, certain chemical bonds break, and new ones form. For example, when a glucose molecule breaks down, bonds between the molecule's carbon atoms break. Since these are energy-storing bonds, they release energy when broken. However, the molecule must be somewhat contorted to get into a state that allows the bonds to...
23.0K
Enzymes02:34

Enzymes

Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
94.1K
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
86.4K
Phosphate Buffer01:22

Phosphate Buffer

The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
5.0K
Enzyme Kinetics01:19

Enzyme Kinetics

Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
103.9K
Inertial Frames of Reference01:03

Inertial Frames of Reference

Newton’s first law is usually considered to be a statement about reference frames. It provides a method for identifying a special type of reference frame: the inertial reference frame. In principle, we can make the net force on a body zero. If its velocity relative to a given frame is constant, then that frame is said to be inertial. So, by definition, an inertial reference frame is a reference frame where Newton's first law holds valid. Newton's first law applies to objects with...
8.7K