Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

4.1K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.1K
Ribosomes01:27

Ribosomes

75.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
75.2K
Ribosomes01:27

Ribosomes

10.7K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
10.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

14.7K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
14.7K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

4.3K
4.3K
Taping Over Different Ground Profiles01:12

Taping Over Different Ground Profiles

358
Taping over varying ground profiles requires careful adaptation to achieve accurate measurements. On smooth, level ground with minimal vegetation, the tape can rest directly on the ground. Here, the taping team, typically consisting of a head and a rear tapeman, coordinates their positions with clear communication. The rear tapeman holds the tape at the starting point and guides the head tapeman toward a range pole placed beyond the endpoint, using hand or voice signals to ensure alignment.On...
358

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution single-cell sequencing of trans-spliced mRNA.

Nature protocols·2026
Same author

Genomic determinants of antigen expression hierarchy in African trypanosomes.

Nature·2025
Same author

Inter-chromosomal transcription hubs shape the 3D genome architecture of African trypanosomes.

Nature communications·2024
Same author

SLAM-seq reveals independent contributions of RNA processing and stability to gene expression in African trypanosomes.

Nucleic acids research·2024
Same author

Publisher Correction: Tissue spaces are reservoirs of antigenic diversity for Trypanosoma brucei.

Nature·2024
Same author

Tissue spaces are reservoirs of antigenic diversity for Trypanosoma brucei.

Nature·2024

Related Experiment Video

Updated: Jan 26, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

3.0K

Ribosome Profiling in Trypanosomatids.

Amelie J Kraus1,2,3, Raúl O Cosentino4,5,6

  • 1Department of Veterinary Sciences, Experimental Parasitology, Ludwig-Maximilians-Universität München, Munich, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|April 14, 2019
PubMed
Summary

Ribosome profiling uses deep sequencing to identify protein-coding transcripts and analyze translation rates. This method reveals which messenger RNAs (mRNAs) are actively translated into proteins, offering insights into gene expression and protein synthesis control.

Keywords:
CDSsDeep sequencingGene expressionProtein abundanceRibosome positionsTranslation efficiencyTranslational controlmRNA levels

More Related Videos

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.2K
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

11.9K

Related Experiment Videos

Last Updated: Jan 26, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

3.0K
De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

4.2K
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

11.9K

Area of Science:

  • Molecular Biology
  • Genomics
  • Translational Control

Background:

  • Ribosomes are essential cellular machinery for protein synthesis.
  • Understanding mRNA translation is crucial for comprehending gene expression.
  • Existing methods may not fully capture active protein synthesis rates.

Purpose of the Study:

  • To describe the ribosome profiling technique.
  • To highlight its application in identifying protein-coding transcripts.
  • To demonstrate its utility in analyzing translation efficiency and gene expression.

Main Methods:

  • High-throughput sequencing of ribosome-protected mRNA fragments (footprints).
  • Nuclease digestion to remove non-ribosome-bound RNAs.
  • Deep sequencing to quantify individual footprint abundance.
  • Bioinformatic analysis to determine protein-coding regions and expression levels.

Main Results:

  • Ribosome profiling accurately identifies actively translated mRNA portions.
  • The method allows for quantification of protein synthesis rates.
  • Differential gene expression analysis based on translation efficiency is feasible.

Conclusions:

  • Ribosome profiling is a powerful tool for studying translation.
  • It provides insights into translational control mechanisms.
  • This technique enhances the understanding of gene expression regulation at the translational level.