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Updated: Jan 26, 2026

Detection of Protein Ubiquitination
Published on: August 19, 2009
A Combined Chemical Derivatization/Mass Spectrometric Method for the Enhanced Detection and Relative Quantification
Navin Chicooree1, John R Griffiths2
1MS-Insight Ltd, Manchester, UK.
Abstract:
Mass spectrometry (MS) is a sensitive analytical technique with wide application across the sciences including for the detection of peptides and proteins in biological analysis. Ubiquitinated (Ub) proteins are typically analyzed by proteolytic digestion and subsequent chromatographic separation followed by MS detection of the resulting isopeptides. Here we describe a novel method which enables enhanced detection of this important posttranslational modification (PTM) by use of a simple chemical labeling strategy prior to Data-Independent Acquisition (DIA) using a SWATH-based acquisition approach on a suitable Quadrupole-Time-Of-Flight (Q-TOF) mass spectrometer.
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