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Updated: Jan 26, 2026

Nucleoside Triphosphates - From Synthesis to Biochemical Characterization
Published on: April 3, 2014
Synthesis and Biochemical Evaluation of an Artificial, Fluorescent Glucosinolate (GSL)
Carina Patrizia Glindemann1, Anita Backenköhler2, Matthias Strieker2
1Institute of Organic Chemistry, Technische Universität Carolo Wilhelmina zu Braunschweig, Hagenring 30, 38106, Braunschweig, Germany.
Abstract:
The synthesis of the first example of a fluorescent glucosinolate (GSL)-BODIPY conjugate based on an azide-containing artificial GSL precursor (GSL-N3 ) is reported. Biochemical evaluation of the artificial GSLs revealed that the compounds are converted to the corresponding isothiocyanates in the presence of myrosinase. Furthermore, myrosinase-catalyzed hydrolysis in the presence of plant specifier proteins yielded the expected alternative products, namely nitriles. The easy assembly of the fluorescent GSL-BODIPY conjugate by click chemistry from GSL-N3 holds potential for application as a fluorescence labeling tool to investigate GSL-associated processes.
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