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Updated: Jan 26, 2026

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

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Published on: January 7, 2019

Nitric oxide releasing poly(vinylidene fluoride-co-hexafluoropropylene) films using a fluorinated nitric oxide donor

Yang Zhou¹, Jinyi Tan¹, Jianfeng Wu²

¹Department of Chemistry, University of Michigan, Ann Arbor, MI 48109, USA.

Acta Biomaterialia

|April 14, 2019

Summary

This study introduces a novel nitric oxide (NO)-releasing fluoropolymer, PVDF-HFP, with significantly reduced chemical leaching. The new biomaterial demonstrates sustained NO release for 16 days and exhibits potent antimicrobial properties.

Keywords:

Antibacterial activity Chemical leaching Fluoropolymer Nitric oxide releasing Poly(vinylidene fluoride-co-hexafluoropropylene)

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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Area of Science:

Biomaterials Science
Polymer Chemistry
Medical Devices

Background:

Nitric oxide (NO) releasing polymers are crucial biomaterials for medical implants.
Leaching of NO donors and byproducts from conventional polymers limits their application.
Fluoropolymers are widely used in biomedical devices but NO-releasing variants are underexplored.

Purpose of the Study:

To develop a novel NO-releasing fluoropolymer with minimal chemical leaching.
To create a stable NO-releasing poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) copolymer.
To evaluate the NO release duration, leaching levels, and antimicrobial efficacy of the new material.

Main Methods:

Synthesized a PVDF-HFP copolymer incorporating a fluorinated S-nitrosothiol as an NO donor.
Measured NO release under physiological conditions over time.
Quantified the leaching of the NO donor and its disulfide byproduct.
Assessed antimicrobial and anti-biofilm activity against S. aureus and P. aeruginosa.
Coated Teflon tubing with the NO-releasing polymer.

Main Results:

The developed PVDF-HFP films released NO for 16 days under physiological conditions.
Total chemical leaching of the NO donor and its byproduct was only 0.6% after 9 days.
The NO-releasing PVDF-HFP films demonstrated significant antimicrobial and anti-biofilm activity.
Coating Teflon tubing with the polymer enabled sustained NO release.

Conclusions:

A novel, stable NO-releasing PVDF-HFP copolymer was successfully prepared.
The material exhibits greatly reduced chemical leaching, enhancing biocompatibility.
The NO-releasing PVDF-HFP copolymer shows promise for improving biomedical devices with antimicrobial properties.