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Dual-functionalized hyaluronic acid as a facile modifier to prepare polyanionic collagen
Min Zhang1, Junhui Yang1, Feng Deng1
1College of Materials Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Carbohydrate Polymers
|April 15, 2019
Summary
Hyaluronic acid (HA) was modified into HA-N-hydroxysuccinimide (NHS) esters, creating a novel collagen modifier. This modification yields water-soluble collagen with enhanced thermal stability, expanding applications in biomaterials and cosmetics.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Biochemistry
Background:
- Hyaluronic acid (HA) is a natural polysaccharide with significant physiological activities.
- Collagen modification is crucial for enhancing its properties and expanding its applications.
- Developing novel modifiers for collagen is essential for advanced biomaterial development.
Purpose of the Study:
- To synthesize N-hydroxysuccinimide (NHS) esters of hyaluronic acid (HA) as a novel collagen modifier.
- To investigate the structural, solubility, and thermal properties of collagen modified with HA-NHS esters.
- To explore the potential applications of modified collagen in biomaterials and cosmetics.
Main Methods:
- Esterification reaction to synthesize HA-NHS esters.
- Fourier transform infrared spectroscopy (FTIR) and 1H-nuclear magnetic resonance (NMR) for structural characterization.
- Circular dichroism (CD) and SDS-PAGE to assess collagen structure and molecular weight.
- Solubility tests, isoelectric point determination, thermal analysis, FESEM, and rheological tests to evaluate properties.
Main Results:
- Successful synthesis of HA-NHS esters confirmed by FTIR and NMR.
- Modified collagen retained its triplex structure with increased molecular weight, as shown by FTIR, CD, and SDS-PAGE.
- HA-NHS ester modification significantly decreased the isoelectric point, resulting in water-soluble collagen.
- Thermal transition temperature of collagen increased by 16°C.
- Aggregation morphology and rheological properties were dependent on the NHS/COOH ratio.
Conclusions:
- HA-NHS esters serve as effective dual-functional modifiers for collagen.
- Modified collagen exhibits enhanced water solubility and thermal stability.
- This approach offers a pathway to tailor collagen properties for applications in injectable biodegradable materials and cosmetics.
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