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05:30
Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
Published on: November 8, 2024
912
Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity
Masashi Ueda1, Yukiyasu Sato2, Akihito Horie1
1Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, 606-8507, Japan.
Molecular and Cellular Endocrinology
|April 15, 2019
Summary
Extravillous trophoblasts (EVTs) use CD59 to evade complement-dependent cytotoxicity (CDC) in the maternal blood. This study shows CD59 protects endovascular trophoblasts from complement attack during human placenta development.
Area of Science:
- Reproductive biology
- Immunology
- Cell biology
Background:
- Extravillous trophoblasts (EVTs) invade maternal tissues during human placenta formation.
- Endovascular trophoblasts are exposed to maternal complement but resist complement-dependent cytotoxicity (CDC).
Purpose of the Study:
- To investigate the expression and function of CD59, a complement regulator, in EVTs.
Main Methods:
- Immunohistochemistry of early human implantation sites.
- CD59 gene silencing in the human EVT-like cell line Swan71.
- In vitro complement-dependent cytotoxicity (CDC) and apoptosis assays.
Main Results:
- CD59 expression was high on endovascular trophoblasts but low on interstitial trophoblasts.
- CD59-silenced EVT-like cells showed increased susceptibility to CDC.
- EVT-like cells with silenced CD59 exhibited higher apoptosis rates.
Conclusions:
- CD59 is expressed on endovascular trophoblasts, suggesting a role in protecting them from maternal complement.
- CD59 likely confers protection against complement-mediated attack to endovascular trophoblasts, facilitating successful pregnancy.
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