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Updated: Jan 26, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Supercritical-Fluid Chromatography On-Chip with Two-Photon-Excited-Fluorescence Detection for High-Speed Chiral
Josef J Heiland1, David Geissler1, Sebastian K Piendl1
1Institute of Analytical Chemistry , Leipzig University , Linnéstraße 3 , 04103 Leipzig , Germany.
Abstract:
Herein, we present the first example of microchip-based supercritical-fluid chromatography (SFC). A microfluidic-glass-chip platform with pressure and temperature control for fast and efficient on-column injection is described. This enabled fast and efficient separation of chiral and achiral compounds within seconds and also employed two-photon-excitated-fluorescence detection. Peak shapes were highly regular and symmetric even for linear flow rates over the packed microchip column in a range of up to 20 mm·s-1.
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