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Related Experiment Video

Updated: Jan 26, 2026

Assessing Cerebral Autoregulation via Oscillatory Lower Body Negative Pressure and Projection Pursuit Regression
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Assessing Cerebral Autoregulation via Oscillatory Lower Body Negative Pressure and Projection Pursuit Regression

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WRINKLED1 Is Subject to Evolutionary Conserved Negative Autoregulation.

Per Snell1, Åsa Grimberg1, Anders S Carlsson1

  • 1Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.

Frontiers in Plant Science
|April 16, 2019
PubMed
Summary

WRINKLED1 (WRI1) transcription factors, crucial for plant oil production, are negatively regulated by an indirect feedback loop. This autoregulation can be overcome by creating modified WRI1 versions for agricultural biotechnology.

Keywords:
fluorescent electrophoretic mobility shift assaygene circuitgene regulationgenetic engineeringintrinsically disordered regionprotein engineeringself-regulated gene

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Agricultural Science

Background:

  • Accumulation of storage compounds like oil and starch is vital for crop value.
  • WRINKLED1 (WRI1) is a key transcription factor regulating carbon allocation into plant oil.
  • Understanding WRI1's genetic network is crucial for crop improvement.

Purpose of the Study:

  • To investigate the autoregulatory system of WRINKLED1 (WRI1).
  • To identify the mechanisms and components involved in WRI1 autoregulation.
  • To explore the potential for modifying WRI1 for biotechnological applications.

Main Methods:

  • Transient expression assays in *Nicotiana benthamiana* leaves.
  • GUS reporter gene assays with varying lengths of the *Arabidopsis WRI1* promoter.
  • Expression of WRI1 homologs from diverse plant species.
  • Structural analysis and domain swapping between oat and *Arabidopsis* WRI1.
  • DNA/protein interaction assays.

Main Results:

  • Increasing WRI1 homolog levels reduced the transcriptional activity of the *Arabidopsis WRI1* promoter.
  • Negative autoregulation was dependent on the DNA-binding AP2-domains of WRI1.
  • AtWRI1 did not directly bind to its upstream regulatory region, indicating indirect interaction.
  • Semi-synthetic WRI1 homologs could bypass the negative autoregulation.

Conclusions:

  • A negative feedback loop regulates *WRI1* gene expression indirectly.
  • The AP2-domain is critical for this autoregulation.
  • Modified WRI1 homologs offer potential for enhanced crop oil production through biotechnology.