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Determining Specific Thyroid Transcripts in Peripheral Blood: A Single Center Study Experience.
Makazlieva T1, Eftimov A2, Vaskova O1
1Institute of Pathophysiology and Nuclear Medicine, Medical Faculty, Skopje, Republic of Macedonia.
Balkan Journal of Medical Genetics : BJMG
|April 16, 2019
Summary
Thyroid carcinoma (TC) detection may be improved by measuring thyrotropin receptor (TSHR)-mRNA in blood. Elevated TSHR-mRNA levels indicate recurrence or metastasis in differentiated TC patients.
Area of Science:
- Molecular biology
- Oncology
- Biomarker discovery
Background:
- Thyroid carcinoma (TC) presents diverse prognoses.
- Molecular diagnostics offer potential for metastatic TC detection.
Purpose of the Study:
- To analyze messenger RNA (mRNA) expression of the thyrotropin receptor (TSHR) and thyroglobulin (Tg) genes in peripheral blood.
- To evaluate these genes as biomarkers for differentiated TC in patients versus healthy individuals.
Main Methods:
- Peripheral blood RNA isolation from 40 TC patients and 17 healthy controls.
- Two-step reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR.
- Relative quantification using the 2-ΔΔCt method.
Main Results:
- TSHR-mRNA was significantly elevated (8.57-fold) in metastatic/relapsed TC patients compared to controls.
- Biochemical relapse showed a 14.17-fold increase in TSHR-mRNA.
- Tg-mRNA was higher (6.6-fold) in metastatic/relapsed patients versus controls.
Conclusions:
- TSHR-mRNA expression in peripheral blood shows promise as a sensitive biomarker for differentiated TC.
- TSHR-mRNA may be more advantageous than Tg-mRNA for detecting TC recurrence and metastasis.
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