Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Active E rosette formation by human lymphoblasts.

G Semenzato, G Amadori, P Sarasin

    Immunology
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Recognizing megatsunamis in Mediterranean deep sea sediments based on the massive deposits of the 365 CE Crete event.

    Scientific reports·2022
    Same author

    The Dallol Geothermal Area, Northern Afar (Ethiopia)-An Exceptional Planetary Field Analog on Earth.

    Astrobiology·2019
    Same author

    Diagnostic and prognostic value of low percentage of glycosylated ferritin in acquired hemophagocytic lymphohistiocytosis: A single-center study.

    International journal of laboratory hematology·2017
    Same author

    Protein kinase CK2 regulates AKT, NF-κB and STAT3 activation, stem cell viability and proliferation in acute myeloid leukemia.

    Leukemia·2016
    Same author

    Targeting CK2-driven non-oncogene addiction in B-cell tumors.

    Oncogene·2016
    Same author

    Early effects of the antineoplastic agent salinomycin on mitochondrial function.

    Cell death & disease·2015
    Same journal

    Epidermal Dominance of Metabolically Constrained Immune Niches Underpins Immune Activation Signatures and Clinical Severity in Psoriatic Disease.

    Immunology·2026
    Same journal

    Thymic APC Networks Orchestrate T-Cell Selection: Mechanisms and Therapeutic Opportunities in Immune Disorders.

    Immunology·2026
    Same journal

    Immune Checkpoints in Leukaemia as Gatekeepers of Immuno-Modulation.

    Immunology·2026
    Same journal

    Senescent Human Liver Endothelial Cells Mediate CD4<sup>+</sup> T Cell Recruitment via ICOSL.

    Immunology·2026
    Same journal

    The Transcription Factor EGR2 Plays a Central Role in the Expansion and Function of TCRαβ<sup>+</sup>CD4<sup>-</sup>CD8<sup>-</sup> Double Negative T Cells in lpr Lupus Mice.

    Immunology·2026
    Same journal

    Epigenetic Gene Networks Governing Immune State Transitions Across the Lifespan.

    Immunology·2026
    See all related articles

    Active E rosettes, a specific lymphocyte subpopulation, increase with phytohaemagglutinin (PHA) stimulation. This indicates active E rosettes represent functionally active T cells in blood.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lymphocytes play a crucial role in the immune system.
    • E rosettes are formed by lymphocytes binding to sheep red blood cells.
    • Phytohaemagglutinin (PHA) is a lectin that stimulates T lymphocytes.

    Purpose of the Study:

    • To evaluate the percentage of active and total E rosettes in lymphocytes.
    • To determine the effect of phytohaemagglutinin (PHA) on E rosette formation.
    • To investigate if active E rosettes represent a specific subpopulation of T cells.

    Main Methods:

    • Culturing lymphocytes from 20 normal subjects for 72 hours.
    • Stimulating lymphocytes with phytohaemagglutinin (PHA).
    • Quantifying active and total E rosettes in both stimulated and unstimulated lymphocytes.

    Related Experiment Videos

    Main Results:

    • A significant increase in active E rosette formation was observed in PHA-stimulated lymphocytes compared to unstimulated lymphocytes.
    • The percentage of total E rosettes also showed changes with PHA stimulation.
    • The findings suggest a distinct behavior of active E rosettes under stimulation.

    Conclusions:

    • Active E rosettes represent a specific subpopulation of lymphocytes.
    • These cells are functionally active T cells present in peripheral blood.
    • PHA stimulation enhances the formation of active E rosettes, confirming their role as activated T cells.