Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:19

Cell Migration

6.5K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
6.5K
Cell Migration01:09

Cell Migration

18.7K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
18.7K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

3.2K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
3.2K
Chemotaxis and Direction of Cell Migration01:21

Chemotaxis and Direction of Cell Migration

4.5K
Cells can detect chemical cues in their environment and reorganize the cytoskeleton to migrate toward them or away from them. This directional migration, called chemotaxis, is essential during embryogenesis and development, immune response, tissue repair and regeneration, and reproduction. These chemical cues can either attract or repel the cell's movement. For example, axon development is determined by a combination of chemoattractants and chemorepellents that direct the growing axon...
4.5K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

3.2K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.2K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.5K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neurosurgical Endovascular Credentialing in Europe and the United Kingdom for the "Complete" Neurovascular Surgeon: The Time has Come.

Neurosurgery·2026
Same author

IDH1-R132H enhances oncolytic HSV-1 therapy by facilitating viral entry and immune activation in glioma.

Nature communications·2026
Same author

Publisher Correction: Tumor transcriptional state predicts survival in immune-checkpoint-blockade-treated glioblastoma.

Nature cancer·2026
Same author

Tumor transcriptional state predicts survival in immune-checkpoint-blockade-treated glioblastoma.

Nature cancer·2026
Same author

Biomarker-Guided Strategies for Tumor Vasculature: From Imaging Advances to Novel Therapeutic Interventions.

Cancer letters·2026
Same author

Treatment Effect Reanalysis of the Randomized Individual Screening Trial of Innovative Glioblastoma Therapy in Newly Diagnosed Glioblastoma With External Control Data.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology·2026

Related Experiment Video

Updated: Jan 26, 2026

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
09:36

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Published on: August 29, 2018

10.8K

Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration.

Michal O Nowicki1, Josie L Hayes2, E Antonio Chiocca3

  • 1Harvey W. Cushing Neurooncology Laboratories, Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. mnowicki@bwh.harvard.edu.

Cancers
|April 17, 2019
PubMed
Summary

Inhibiting glycogen synthase kinase-3 (GSK-3) blocks glioblastoma (GBM) cell migration by reducing vimentin. This impacts vimentin dynamics and GBM cell invasion.

Keywords:
GSK-3cytoskeletonglioblastomamotilityvimentin

More Related Videos

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

6.8K
Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain
09:29

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Published on: July 29, 2022

3.2K

Related Experiment Videos

Last Updated: Jan 26, 2026

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion
09:36

Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion

Published on: August 29, 2018

10.8K
Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions
10:08

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Published on: February 24, 2021

6.8K
Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain
09:29

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Published on: July 29, 2022

3.2K

Area of Science:

  • Oncology
  • Cell Biology
  • Biochemistry

Background:

  • Glioblastoma (GBM) cell migration is a key factor in tumor invasion.
  • Glycogen synthase kinase-3 (GSK-3) inhibitors, like lithium chloride (LiCl), have shown potential in blocking GBM cell migration.

Purpose of the Study:

  • To investigate the molecular mechanisms by which GSK-3 inhibitors affect GBM cell migration.
  • To identify key proteins altered by GSK-3 inhibition in GBM cells.

Main Methods:

  • Two-dimensional difference in-gel electrophoresis (2D-DIGE) and mass spectrometry were used to identify protein changes.
  • Vimentin expression and function were analyzed using siRNA knockdown, Western blotting, and Fluorescence Recovery After Photobleaching (FRAP) microscopy.
  • Direct interaction between GSK-3 and vimentin was assessed biochemically.

Main Results:

  • Downregulation of the intermediate filament protein vimentin was the most significant change observed after LiCl treatment.
  • Vimentin is highly expressed in GBM tumors and is prognostic for patient outcomes.
  • Vimentin knockdown and GSK-3 inhibition reduced GBM cell migration and altered vimentin cytoskeletal dynamics.

Conclusions:

  • GSK-3 directly interacts with and phosphorylates vimentin.
  • Inhibiting GSK-3 impacts vimentin cytoskeletal dynamics, contributing to the anti-migratory effects of these compounds in GBM.