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Related Experiment Videos

Tau protein function in living cells.

D G Drubin, M W Kirschner

    The Journal of Cell Biology
    |December 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Tau protein, a microtubule-associated protein, was microinjected into cells lacking it. In vivo, tau protein promotes tubulin assembly and stabilizes microtubules without altering cell structure.

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    Area of Science:

    • Neuroscience
    • Cell Biology

    Background:

    • Tau protein promotes microtubule polymerization in vitro.
    • Its in vivo effects on tubulin assembly are unknown.
    • Tau protein is induced during nerve cell differentiation.

    Purpose of the Study:

    • To investigate the in vivo activity of tau protein on tubulin assembly.
    • To determine if tau protein affects microtubule polymerization within cells.

    Main Methods:

    • Microinjection of tau protein into fibroblast cells lacking endogenous tau.
    • Immunofluorescence to visualize tau protein localization.
    • Assessment of tubulin polymerization and microtubule stability.

    Main Results:

    • Tau protein microinjected into fibroblasts specifically associated with microtubules.

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  • Injected tau protein increased tubulin polymerization in vivo.
  • Microtubules were stabilized against depolymerization by tau protein.
  • No significant changes in cell morphology or microtubule arrangement were observed.

    • Conclusions:

    • Tau protein acts in vivo to induce tubulin assembly and stabilize microtubules.
    • These activities are potentially necessary, but not sufficient, for neuronal morphogenesis.