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Related Experiment Video

Updated: Jan 26, 2026

Minimally Invasive Isolated Limb Perfusion (MI-ILP) for Locally Advanced Melanomas and Sarcomas of the Extremity
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Published on: January 31, 2025

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BRAF status as a predictive factor for response in isolated limb perfusion.

Valerio Belgrano1,2, Jan Mattsson1,3, Jonas Nilsson2

  • 1a Department of Surgery , Institute of Clinical Sciences, Sahlgrenska Academy at the University of Gothenburg , Gothenburg , Sweden.

International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group
|April 17, 2019
PubMed
Summary

The BRAF V600E/K mutation does not predict treatment response or survival in patients undergoing isolated limb perfusion (ILP) for in-transit melanoma metastases. This finding suggests other factors may influence outcomes in this patient group.

Keywords:
BRAF mutationMalignant melanomaimmunotherapyin-transit metastasesisolated limb perfusionsurvival

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Area of Science:

  • Oncology
  • Surgical Oncology
  • Melanoma Research

Background:

  • Isolated limb perfusion (ILP) is a key treatment for unresectable in-transit melanoma metastases.
  • Tumor burden is a known predictor of response to ILP.
  • Identifying additional predictive factors can optimize patient selection and treatment outcomes.

Purpose of the Study:

  • To investigate the predictive value of BRAF V600E/K mutation status for treatment response and survival in patients undergoing ILP for melanoma.
  • To determine if BRAF mutation status can identify subgroups with higher response rates.

Main Methods:

  • Retrospective analysis of 98 consecutive patients undergoing first-time ILP with melphalan for melanoma in-transit metastases (2012-2017).
  • Assessment of tumor burden (lesion number, largest diameter) and BRAF V600E/K mutation status.
  • Classification of response rates using World Health Organization (WHO) criteria.

Main Results:

  • 33% of patients (32/98) harbored the BRAF V600E/K mutation; 66% (66/98) were wild type (wt).
  • No significant differences in response rates (overall: 69% vs. 77%; complete: 47% vs. 52%) or median survival (47 months) between BRAF mutated and wt groups.
  • Tumor burden and BRAF status showed no significant difference between groups.

Conclusions:

  • BRAF V600E/K mutation status is not a significant predictor of response or survival following isolated limb perfusion for in-transit melanoma metastases.
  • Further research is needed to identify other biomarkers that may predict ILP outcomes.