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Related Concept Videos

Development of Analytical Methods01:21

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An analytical methodology can be divided into four sequential steps: technique, method, procedure, and protocol. A technique is a scientific principle that rationalizes a specific phenomenon through chemical measurements. Adapting a technique for analyzing a sample of interest is termed a method. The procedure outlines the directions for performing the analysis via an analytical method. The protocol is the detailed guidelines on the procedure, which should be strictly followed to obtain the...
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Although not a source of energy, cholesterol plays a significant role as a foundational structure for bile salts, steroid hormones, and vitamin D, as well as being a crucial component of plasma membranes. Approximately 15% of blood cholesterol is derived from our diet, with the remainder synthesized from acetyl CoA by the liver and intestines. Cholesterol is eliminated from the body through its conversion into bile salts, which are eventually discarded in the feces.
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In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
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Carl Jung, a Swiss psychiatrist and former follower of Freud, eventually broke away from Freud's ideas to create his framework, analytical psychology. This approach emphasizes achieving a balance between the conscious and unconscious aspects of the mind and reconciling various experiences within an individual's personality. Jung believed that this process, which typically unfolds in the latter part of life, involves an ongoing journey of recognizing and incorporating unconscious...
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Graphical and Analytic Representation of Sinusoids01:20

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Analyzing two sinusoidal voltages with equal amplitude and period but different phases on an oscilloscope, an instrument used to display and analyze waveforms, involves a three-step process.
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When drugs are administered, they can elicit either an agonist or antagonist effect on the body. Agonism occurs when a drug activates a specific receptor, triggering a biological response. On the other hand, antagonism happens when a drug binds to the same receptors but blocks their activation, thereby preventing a biological response.
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Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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Analytical methods for cholesterol quantification.

Li-Hua Li1, Ewelina P Dutkiewicz2,3, Ying-Chen Huang2

  • 1Department of Pathology and Laboratory Medicine, Taipei Veterans General Hospital, Taipei, Taiwan.

Journal of Food and Drug Analysis
|April 17, 2019
PubMed
Summary
This summary is machine-generated.

Accurate cholesterol measurement is vital for diagnosing diseases. This review covers prevalent and emerging methods, including ambient ionization mass spectrometry (AIMS), for cholesterol quantification in biological samples and foods.

Keywords:
AIMSCholesterolDARTDESIMass spectrometryQuantification

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Cholesterol is a critical lipid in cell membranes and a precursor for vital biomolecules.
  • Abnormal cholesterol levels are linked to numerous diseases, including cardiovascular and metabolic disorders.
  • Accurate cholesterol quantification is essential for disease risk assessment and management.

Purpose of the Study:

  • To review established and novel methods for cholesterol quantification.
  • To highlight advanced techniques like ambient ionization mass spectrometry (AIMS) for rapid analysis.
  • To present molecular imaging applications for cholesterol in biological tissues.

Main Methods:

  • Classical chemical assays
  • Enzymatic assays
  • Chromatographic techniques (GC, LC)
  • Mass spectrometry (MS)
  • Ambient ionization mass spectrometry (AIMS)

Main Results:

  • A comprehensive overview of prevalent cholesterol quantification methods is provided.
  • Ambient ionization mass spectrometry (AIMS) emerges as a promising next-generation platform for rapid, in situ analysis.
  • Molecular imaging techniques offer new possibilities for visualizing cholesterol distribution in tissues.

Conclusions:

  • The review consolidates current knowledge on cholesterol measurement techniques.
  • Emerging technologies like AIMS offer significant advantages for cholesterol analysis.
  • Future research should focus on refining and validating these advanced methods for clinical and food analysis.