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Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
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Gene expression profile in mouse bacterial chronic rhinosinusitis
Liang Geng1,2, Sang Wang1, Yanyan Zhao3
1Department of Otolaryngology, Shanghai General Hospital, Shanghai Jiaotong University, Hongkou, Shanghai 200080, P.R. China.
Experimental and Therapeutic Medicine
|April 17, 2019
Summary
Persistent Staphylococcus aureus infection causes bacterial chronic rhinosinusitis, leading to severe inflammation and gene changes. Coagulation cascade pathways are significantly upregulated in this condition.
Area of Science:
- Microbiology
- Immunology
- Pathogenesis
Background:
- Persistent bacterial infections in paranasal sinuses impair sinus drainage, leading to bacterial chronic rhinosinusitis (BCRS).
- Understanding key molecular players in BCRS pathogenesis is crucial for developing effective treatments.
Purpose of the Study:
- To investigate gene expression profiles and analyze key pathways involved in the pathogenesis of BCRS.
- To identify differentially expressed genes and associated pathways in a mouse model of BCRS.
Main Methods:
- BCRS was induced in mice using Staphylococcus aureus-inoculated Merocel nasal packs.
- Mucosal samples were collected for histological, ELISA, and DNA microarray analysis.
- Gene expression data underwent pathway and gene ontology analysis.
Main Results:
- BCRS induced significant epithelial damage, lamina propria edema, and mucosal inflammation.
- 6,018 genes were differentially expressed in BCRS mice compared to controls.
- Upregulation of plasma, coagulation factors, urokinase plasminogen activator, and urokinase receptor plasminogen activator was observed.
Conclusions:
- BCRS is characterized by severe mucosal inflammation and significant gene expression alterations.
- The coagulation cascade pathways are upregulated in the pathogenesis of BCRS.
- These findings highlight the role of coagulation in BCRS and suggest potential therapeutic targets.
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