Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Urinary Bladder01:23

Urinary Bladder

3.3K
The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...
3.3K
Anatomy of the Genitourinary System II: Bladder and Urethra01:19

Anatomy of the Genitourinary System II: Bladder and Urethra

1.5K
The lower urinary system consists of the urinary bladder and urethra, which are essential in storing and expelling urine from the body. Together with the internal and external sphincters, these structures work together to regulate urination effectively.Anatomy of the BladderThe urinary bladder is a muscular, stretchable organ behind the pubic bone and in front of the rectum. In females, the bladder is positioned anterior to the vagina and inferior to the uterus, while in males, it is located...
1.5K
Imaging Studies I: Kidney, Ureter, and Bladder Studies01:28

Imaging Studies I: Kidney, Ureter, and Bladder Studies

317
Kidney, Ureter, and Bladder (KUB) StudiesKidney, Ureter, and Bladder (KUB) studies are standard diagnostic imaging procedures used to assess the anatomy of the urinary system. They are commonly utilized for patients experiencing abdominal pain or urinary symptoms. By using a simple X-ray of the abdomen, KUB studies can reveal structural and pathological abnormalities within the kidneys, ureters, and bladder. These studies are particularly valuable in diagnosing kidney stones, urinary...
317
Seed Structure and Early Development of the Sporophyte02:33

Seed Structure and Early Development of the Sporophyte

31.0K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
31.0K
Adult Stem Cells01:33

Adult Stem Cells

33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.1K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Friend and Confidant Thresholds: Social Network Size as a Mediator Between Marital Status and Major Depressive Disorder.

Depression and anxiety·2026
Same author

Picropodophyllotoxin Mitigates Severe Inflammation Through HMGB1 Inhibition.

Biomolecules·2026
Same author

Development of Pentaplex Reverse Transcription Droplet Digital PCR Assay for Simultaneous Detection and Absolute Quantification of HIV-1, HIV-2, HCV, and HBV With Internal Control.

Electrophoresis·2026
Same author

Analysis of unmapped RNA-seq data from cancer spatial transcriptome toward characterizing cancer microbiome.

Scientific reports·2026
Same author

Niraparib With Abiraterone Acetate Plus Prednisone as First-Line Therapy in Patients With Metastatic Castration-Resistant Prostate Cancer With Homologous Recombination Repair Gene Alterations: Final Analysis of the Asian Subgroup From the MAGNITUDE Study.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

Forensic DNA extraction from decomposed human soft tissues: Optimization using ethanol treatment and surfactant-based lysis conditions.

Forensic science international·2026

Related Experiment Video

Updated: Jan 26, 2026

Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds
09:18

Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds

Published on: July 16, 2018

8.7K

Stem Cells Seeded on Multilayered Scaffolds Implanted into an Injured Bladder Rat Model Improves Bladder Function.

Kshitiz Raj Shrestha1,2, Seung Hwan Jeon1, Ae Ryang Jung1,2

  • 11Department of Urology, College of Medicine, Seoul St. Mary's Hospital, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, 06591 Republic of Korea.

Tissue Engineering and Regenerative Medicine
|April 17, 2019
PubMed
Summary

Human adipose-derived stem cells (hADSCs) on poly(l-lactide-co-ɛ-caprolactone) (PLCL) sheets improved bladder function in rats. These stem cells differentiated into smooth muscle cells, enhancing bladder compliance and contractility.

Keywords:
ComplianceSmooth muscleStem cellsTissue engineeringUrinary bladder

More Related Videos

An Orthotopic Model of Murine Bladder Cancer
09:07

An Orthotopic Model of Murine Bladder Cancer

Published on: February 6, 2011

16.5K
Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice
11:46

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

Published on: April 27, 2014

18.3K

Related Experiment Videos

Last Updated: Jan 26, 2026

Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds
09:18

Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds

Published on: July 16, 2018

8.7K
An Orthotopic Model of Murine Bladder Cancer
09:07

An Orthotopic Model of Murine Bladder Cancer

Published on: February 6, 2011

16.5K
Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice
11:46

Urinary Bladder Distention Evoked Visceromotor Responses as a Model for Bladder Pain in Mice

Published on: April 27, 2014

18.3K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Urology

Background:

  • Bladder dysfunction following detrusor smooth muscle removal presents a significant clinical challenge.
  • Current treatments for bladder injury lack effective regenerative strategies.
  • Investigating novel biomaterials and cell-based therapies is crucial for bladder repair.

Purpose of the Study:

  • To evaluate the efficacy of human adipose-derived stem cells (hADSCs) cultured on multilayered poly(l-lactide-co-ɛ-caprolactone) (PLCL) scaffolds for improving bladder function.
  • To assess the potential of hADSCs to differentiate into smooth muscle cells within a damaged bladder environment.

Main Methods:

  • A rat model with surgically removed detrusor smooth muscle was established.
  • Rats were divided into Normal, Injury, PLCL scaffold only, and PLCL scaffold with hADSCs groups.
  • Functional, histological, immunohistochemical, and immunoblot analyses were conducted four weeks post-treatment.

Main Results:

  • Human adipose-derived stem cells demonstrated compatibility with PLCL scaffolds.
  • The PLCL scaffold with hADSCs group exhibited significant improvements in bladder compliance and contractility.
  • Histological analyses confirmed uniform hADSC distribution and differentiation into smooth muscle cells, evidenced by smooth muscle marker expression.

Conclusions:

  • Human adipose-derived stem cells seeded on multilayered PLCL sheets possess the potential for smooth muscle cell differentiation.
  • This approach facilitates the recovery of compliance and contractility in injured bladders.
  • hADSCs on PLCL scaffolds represent a promising regenerative strategy for bladder reconstruction.