Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cobalt blocks the decrease in MEPSP frequency on depolarization in calcium-free hypertonic media.

L Landò, J Giovannini, R S Zucker

    Journal of Neurobiology
    |November 1, 1986
    PubMed
    Summary

    Hyperosmotic sucrose solutions increase spontaneous neurotransmitter release. Cobalt ions block this effect and also block calcium efflux during depolarization, suggesting a dual role for calcium channels in neurotransmission.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Pseudohypopyon in anterior segment neovascularization.

    Journal francais d'ophtalmologie·2020
    Same author

    Idiopathic choroidal folds.

    Journal francais d'ophtalmologie·2020
    Same author

    Presynaptic effectors contributing to cAMP-induced synaptic potentiation in Drosophila.

    Journal of neurobiology·2005
    Same author

    Direct determination of phosphate esters in concentrated nitrate media by capillary zone electrophoresis.

    Journal of chromatography. A·2003
    Same author

    Roles for mitochondrial and reverse mode Na+/Ca2+ exchange and the plasmalemma Ca2+ ATPase in post-tetanic potentiation at crayfish neuromuscular junctions.

    The Journal of neuroscience : the official journal of the Society for Neuroscience·2001
    Same author

    Phosphorylation and local presynaptic protein synthesis in calcium- and calcineurin-dependent induction of crayfish long-term facilitation.

    Neuron·2001

    Area of Science:

    • Neuroscience
    • Cellular Biology
    • Biophysics

    Background:

    • Hyperosmotic conditions and depolarization influence neurotransmitter release.
    • Calcium ions play a critical role in synaptic transmission.

    Purpose of the Study:

    • To investigate the effect of hyperosmotic sucrose on neurotransmitter release.
    • To explore the role of calcium ions and cobalt in modulating this release.

    Main Methods:

    • Measurement of miniature excitatory postsynaptic potentials (MEPSPs) in a calcium-free medium.
    • Depolarization of neurons using high potassium concentrations.
    • Application of cobalt ions to observe their blocking effects.

    Main Results:

    • Hyperosmotic sucrose significantly increased MEPSP frequency.

    Related Experiment Videos

  • Depolarization in calcium-free medium reduced MEPSP frequency.
  • Cobalt ions blocked the reduction in MEPSP frequency caused by depolarization.

    • Conclusions:

    • Hyperosmotic sucrose enhances spontaneous neurotransmitter release.
    • Cobalt ions inhibit calcium efflux during depolarization, suggesting a role in both calcium influx and efflux through calcium channels.