Related Experiment Video

Updated: Jan 26, 2026

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions
04:33

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Published on: March 12, 2019

8.5K

SIRT6 abrogation promotes adrenocortical carcinoma through activation of NF-κB signaling

Xueyi Wu1, Haoming Tian2, Long Xue3

  • 1Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, No. 37, Guoxue Lane, Chengdu, 610041, Sichuan, China.

Molecular and Cellular Biochemistry
|April 17, 2019
PubMed

Insights

Sirtuin 6 (SIRT6) acts as a tumor suppressor in adrenocortical carcinoma (ACC). Inhibiting SIRT6 promotes cancer cell invasion and proliferation by activating the NF-κB pathway, suggesting SIRT6 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Adrenocortical carcinoma (ACC) is a rare cancer with poor prognosis, requiring novel treatments.
  • Sirtuin 6 (SIRT6) is a tumor suppressor involved in regulating cancer cell metabolism and tumorigenesis.

Purpose of the Study:

  • To investigate SIRT6 expression in ACC tissues.
  • To elucidate the role of SIRT6 in generating cancer phenotypes in ACC.

Main Methods:

  • Western blotting, real-time PCR, and immunohistochemistry were used to assess SIRT6 expression.
  • MTT assay, TUNEL assay, and flow cytometry evaluated SIRT6's impact on cell invasion, proliferation, and death.
  • Analysis of downstream signaling pathways including NF-κB, TLR4, CGRP, TRPV1, and reactive oxygen species.

Main Results:

  • SIRT6 expression was found to be inhibited in ACC tissues.
  • SIRT6 knockdown promoted cell invasion, proliferation, and migration while inhibiting cell death.
  • Knockdown of SIRT6 upregulated TLR4, enhanced NF-κB pathway activation, and increased reactive oxygen species generation.

Conclusions:

  • SIRT6 functions as a tumor suppressor in ACC by regulating the NF-κB pathway.
  • Targeting SIRT6 may offer a novel therapeutic strategy for treating adrenocortical carcinoma.
Keywords:
Adrenocortical carcinomaCalcitonin gene-related peptideNF-κBReactive oxygen speciesSirtuin 6Transient receptor potential vanilloid

More Related Videos

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.9K
Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions07:18

Author Spotlight: Manipulating Signaling in Zebrafish Embryos to Decode Cell Fate Decisions

Published on: October 27, 2023

3.3K

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
9.8K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

3.8K
Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
17.2K
Endocrine Signaling01:45

Endocrine Signaling

Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
68.0K
What is Cell Signaling?02:03

What is Cell Signaling?

Despite the protective membrane that separates a cell from the environment, cells need the ability to detect and respond to environmental changes. Additionally, cells often need to communicate with one another. Unicellular and multicellular organisms use a variety of cell signaling mechanisms to communicate to respond to the environment.
130.0K