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Wearable optical resolution photoacoustic microscopy.

Qian Chen1,2, Huikai Xie3, Lei Xi1

  • 1Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, China.

Journal of Biophotonics
|April 17, 2019
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Summary

A new wearable optical resolution photoacoustic microscope (W-ORPAM) allows brain imaging in freely moving rodents. This breakthrough enables studying brain activity and disorders without anesthesia, advancing neuroscience research.

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Optical Imaging

Background:

  • Optical resolution photoacoustic microscopy (ORPAM) is valuable for studying rodent brain activity and disorders, but typically requires anesthesia or restriction.
  • Existing ORPAM systems are not designed for freely moving subjects, limiting in vivo research on natural behaviors and dynamic brain processes.

Purpose of the Study:

  • To develop a wearable and robust optical resolution photoacoustic microscope (W-ORPAM) for imaging brain activity in freely moving rodents.
  • To overcome the limitations of tethered or anesthetized imaging setups in neuroscience research.

Main Methods:

  • Designed and implemented a compact, lightweight, and stable optical scanner for the wearable probe.
  • Achieved high spatiotemporal resolution, a large field of view, and adjustable optical focus for in vivo experiments.
  • Integrated the W-ORPAM system for monitoring cerebral cortex vasculature in a freely moving rat model.

Main Results:

  • The W-ORPAM system demonstrated high performance in imaging cerebral cortex vasculature.
  • Successfully monitored morphological and functional changes in cerebral vasculature during induced ischemia and reperfusion in a freely moving rat.
  • The device proved robust and adaptable for in vivo experiments with freely moving subjects.

Conclusions:

  • The developed W-ORPAM is a significant advancement for studying brain activity and disorders in unanesthetized, freely moving rodents.
  • This technology opens new avenues for real-time, in vivo investigation of neurological conditions and brain dynamics.
  • W-ORPAM offers a powerful, accessible tool for the neuroscience research community.