Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Electric Dipoles and Dipole Moment

Electric Dipoles and Dipole Moment

Consider two charges of equal magnitude but opposite signs. If they cannot be separated by an external electric field, the system is called a permanent dipole. For example, the water molecule is a dipole, making it a good solvent.
Theoretically, studying electric dipoles leads to understanding why the resultant electric forces around us are weak. Since electric forces are strong, remnant net charges are rare. Hence, the interaction between dipoles helps us understand electrical interactions in...

Bond Polarity, Dipole Moment, and Percent Ionic Character

Bond Polarity, Dipole Moment, and Percent Ionic Character

Induced Electric Dipoles

Induced Electric Dipoles

A permanent electric dipole orients itself along an external electric field. This rotation can be quantified by defining the potential energy because the external torque does work in rotating it. Then, the potential energy is minimum at the parallel configuration and maximum at the antiparallel configuration. While the former is a stable equilibrium, the latter is an unstable equilibrium.
Since the absolute value of potential energy holds no physical meaning, its zero value can be chosen as per...

IR Spectrum Peak Intensity: Dipole Moment

IR Spectrum Peak Intensity: Dipole Moment

The dipole moment of a bond is the product of the partial charge on either atom and the distance between them. Dipole moments influence the efficiency of IR absorption and the peak intensity. When a bond with a dipole moment is placed in an electric field, the direction of the field determines if the bond is compressed or stretched. Electromagnetic radiation consists of an electric field component that rapidly reverses direction. It follows that polar bonds are alternately stretched and...

Molecular Geometry and Dipole Moments

Molecular Geometry and Dipole Moments

The VSEPR theory can be used to determine the electron pair geometries and molecular structures as follows:

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

First human decedent model of orthotopic multi-organ xenotransplantation: Whole liver and bilateral kidneys from a six-gene-edited pig.

Med (New York, N.Y.)·2026

Same author

Autonomous Robot-Assisted Video Capture in Open Cardiac Surgery: Surgical Field Detection and Obstacle-Aware Viewpoint Planning via CHELS.

The international journal of medical robotics + computer assisted surgery : MRCAS·2026

Same author

Introduction to bioimaging-based spatial multi-omic novel methods.

Quantitative biology (Beijing, China)·2026

Same author

Single-cell analyses reveal impaired type B spermatogonia differentiation and meiotic entry in C-Nap1-null testes.

Quantitative biology (Beijing, China)·2026

Same author

An integrated AI-enabled system using One Class Twin Cross Learning for early gastric cancer detection.

Frontiers in oncology·2026

Same author

Emerging frontiers in SERS-integrated optical waveguides: advancing portable and ultra-sensitive detection for trace liquid analysis.

Light, science & applications·2025

Same journal

BrainCL: Transformer-Based Brain Network Contrastive Learning with Multi-Order Topology and Salience Masking.

IEEE transactions on medical imaging·2026

Same journal

LLM-enhanced Neuron Segmentation and Reconstruction in Complex Mouse Brain Images.

IEEE transactions on medical imaging·2026

Same journal

Matrixed-Spectrum Decomposition Accelerated Linear Boltzmann Transport Equation Solver for Fast Scatter Correction in Multi-Spectral CT.

IEEE transactions on medical imaging·2026

Same journal

The Ritz Adjoint Method for MRI Pulse Design.

IEEE transactions on medical imaging·2026

Same journal

Physiology-guided Self-supervised Learning for Simultaneous Dual-Tracer PET Separation.

IEEE transactions on medical imaging·2026

Same journal

Informed-Exploration Reinforcement Learning for Automated Virtual Coronary Intervention Planning.

IEEE transactions on medical imaging·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jan 26, 2026

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Published on: April 9, 2017

Group-Sparsity-Based Super-Resolution Dipole Orientation Mapping.

Long Chen, Miaoyan Wang, Xu Zhang

IEEE Transactions on Medical Imaging

|April 17, 2019

Summary

This summary is machine-generated.

A new method, group-sparsity-based super-resolution dipole orientation mapping (GS-SDOM), enhances fluorescence polarization microscopy by improving dipole orientation accuracy. This technique reveals more detailed cellular structures and corrects previous estimation errors.

More Related Videos

Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Related Experiment Videos

Last Updated: Jan 26, 2026

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

Published on: April 9, 2017

Super-resolution Imaging of the Bacterial Division Machinery

Super-resolution Imaging of the Bacterial Division Machinery

Published on: January 21, 2013

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Area of Science:

Biophysics
Cell Biology
Microscopy

Background:

Fluorescence polarization microscopy (FPM) resolves fluorophore dipole orientation for structural insights.
Conventional FPM averages anisotropy within diffraction limits.
Super-resolution dipole orientation mapping (SDOM) improves resolution but has orientation estimation issues.

Purpose of the Study:

To develop an improved SDOM method (GS-SDOM) addressing orientation resolution limitations.
To enhance the accuracy and reliability of dipole orientation mapping.
To extract more detailed morphological and structural information from cellular samples.

Main Methods:

Development of group-sparsity-based SDOM (GS-SDOM).
Utilizing relevance of modulation sequences in the reconstruction model.
Applying sparse deconvolution and least square estimation to fluorescence polarization modulation data.

Main Results:

GS-SDOM achieves higher adjusted R² for dipole orientation, improving credibility.
False positive estimations of local dipole orientation are corrected.
GS-SDOM provides enhanced morphological information and accurate local dipole distribution.

Conclusions:

GS-SDOM significantly improves dipole orientation mapping accuracy and reliability.
The method reveals detailed structures like actin filaments with precise orientation.
GS-SDOM enables advanced 3D co-localization analysis in cellular structures.