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A Small Volume Procedure for Viral Concentration from Water

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Published on: February 3, 2015

Optimal Pyruvate Concentration for the Recovery of Conforms from Food and Water.

Ruby M Lee¹, Paul A Hartman¹

¹Department of Microbiology, Iowa State University, Ames, Iowa 50011-3211.

Journal of Food Protection

|April 18, 2019

Summary

Adding sodium pyruvate to the Violet Red Bile Agar (VRBA) method enhances coliform recovery in water and food samples. The optimal pyruvate concentration for improved coliform detection was determined to be 0.02%.

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Area of Science:

Microbiology
Food Science
Water Quality Analysis

Background:

Coliform detection is crucial for assessing water and food safety.
Standard methods for coliform enumeration may require optimization for enhanced sensitivity.
Violet Red Bile Agar (VRBA) is a common selective medium for coliforms.

Purpose of the Study:

To evaluate the effect of sodium pyruvate on coliform recovery using a Modified VRBA procedure.
To determine the optimal concentration of sodium pyruvate for improved coliform enumeration.

Main Methods:

Coliforms in water and food samples were enumerated using a Modified VRBA procedure.
Tryptic Soy Agar (TSA) base layer and VRBA overlay were utilized.
Six different concentrations of sodium pyruvate (0-1.0%) were tested.
Statistical analysis, including general linear model procedure, was employed.

Main Results:

Sodium pyruvate supplementation significantly increased coliform recoveries compared to controls.
Pyruvate concentrations of 0% and 1.0% resulted in significantly lower counts.
The highest overall counts were observed with 0.05% pyruvate.
No significant differences in counts were found among 0.005%, 0.01%, 0.05%, and 0.1% pyruvate.

Conclusions:

Sodium pyruvate is an effective enhancer for coliform recovery in the Modified VRBA method.
The statistically predicted optimal level of sodium pyruvate for this procedure is 0.02%.