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Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus
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A Collaborative Study Comparing Three ELISA Systems for Detecting Staphylococcus aureus Enterotoxin A in Sausage
H Schönwälder1, J J Haaijman1, R Holbrook1
1Unilever Research Laboratorium Vlaardingen, P.O. Box, 3130 AC Vlaardingen, The Netherlands.
Journal of Food Protection
|April 18, 2019
Summary
Monoclonal antibody ELISA (MCA ELISA) offers reliable staphylococcal enterotoxin A (SEA) detection in food quality control. While quantitative polyclonal antibody ELISA (PCA ELISA) and qualitative FEY ELISA also showed promise, MCA ELISA provided superior sensitivity and specificity.
Area of Science:
- Food safety and analytical chemistry
- Microbiology and immunology
Background:
- Staphylococcal enterotoxin A (SEA) is a significant foodborne pathogen.
- Accurate detection methods are crucial for food quality control.
Purpose of the Study:
- To evaluate three Enzyme-Linked Immunosorbent Assays (ELISAs) for staphylococcal enterotoxin A (SEA) detection.
- To assess their suitability for food quality control laboratories.
Main Methods:
- Collaborative testing of three ELISAs (two quantitative, one qualitative) in five laboratories.
- Evaluation using spiked and unspiked ripened and unripened sausage samples.
- Analysis of sensitivity, reliability, and background responses.
Main Results:
- Quantitative monoclonal antibody ELISA (MCA ELISA) demonstrated higher sensitivity and reliability with lower background responses compared to polyclonal antibody ELISA (PCA ELISA).
- MCA ELISA showed lower enterotoxin recovery, particularly in unripened sausage, but yielded more dependable results at low SEA concentrations (1 μg/100 g).
- The qualitative FEY ELISA provided adequate and reliable results for controlling raw materials and food products.
Conclusions:
- MCA ELISA is a reliable and sensitive method for SEA detection in food quality control.
- FEY ELISA is suitable for routine screening of suspicious food samples.
- Further optimization may be needed for MCA ELISA's enterotoxin recovery in certain food matrices.
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