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Performance of Some Heat-Sensitive Differential Agars Prepared and Melted by Microwave Energy 1
1Department of Animal Sciences and Industry, Kansas State University, Manhattan, Kansas 66506.
Abstract:
The viable cell count performance of some heat-sensitive differential agars prepared and remelted by microwave energy was evaluated for Salmonella choleraesui , Streptococcus faecalis and Escherichia coli . The conventional boiling method was used for comparison. No significant difference was found between the microwave oven processed agar and the conventional-boiling processed agar in viable cell counts of the target bacteria. Heating and reheating of violet red bile agar, bismuth sulfite agar, and KF Streptococcus agar by both methods did not change agar performance. However, remelting of desoxycholate citrate agar by both methods resulted in a substantial lowering of viable cell counts.
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